Fig 1.
Immediate effect of different doses of gamma irradiation (0, 100, 150, 200, 250, 300 and 350 Gy) on seedling parameters of lentil (Lens culinaris Medik.).
(a) Growth status of seedlings after 7 days of experiment. (b) Growth status of seedlings after 14 days of experiment.
Fig 2.
Graphical representation of effect of different doses of acute gamma irradiation (0, 100, 150, 200, 250, 300, and 350 Gy) on (a) gemination percentage at 7 and 14 days after experiment, respectively. (b) seedling vigor index at 7 and 14 days after experiment, respectively.
Fig 3.
Dose response curve of (a) seedling length. (b) root length.
Table 1.
Effect of different doses of gamma radiation on seedling parameters and pollen fertility status in lentils.
Fig 4.
Effect of gamma irradiation on fertility percentage of pollen of lentil (Lens culinaris Medik.) observed under 40X opjective lens of compound light microscope (Olympus OIC); the irradiation doses are: (a) Control (0 Gy). (b) 100 Gy. (c) 150 Gy. (d) 200 Gy. (e) 250 Gy. (f) 300 Gy. (g) 350 Gy; (h) Graphical representation of the same.
Fig 5.
Diagram of development of chloro- and morpho-mutants in M2 generation.
Fig 6.
Detection of chlorophyll and morphological mutants in M2 population.
(a, g, k) Untreated plant, (b-e) Chlorophyll mutants [(b) Albina, (c) Xantha, (d) Chlorina, (e) Xantha-viridis], (f) Chimera, (h-j) Plant morphological mutants, (i-t) Leaf shape (leaf morphological) mutants, (u) Leaf arrangement in untreated plant, (v-w) Change in leaf arrangement in leaf morphological mutants.
Table 2.
Frequency of candidate mutants obtained in the M2 generation.
Table 3.
Agronomic performance of selected M3 mutants: Comparison of plant height, root length, number of pods, and yield with M2 generation and control.
Fig 7.
Selected M3 mutants with improved agronomic characters at 50 DAS than that of control (untreated Moitree).