Fig 1.
Effect of media solidifying agents on strain WR16-4T colony formation.
Data are expressed as mean ± SD (n = 3). N. D., not detected.
Fig 2.
Scanning electron microscopy (SEM) images of strain WR16-4T.
Table 1.
Phenotypic characteristics of strain WR16-4T.
Fig 3.
Assessment of optimal pH for the growth of strain WR16-4T.
OD660 nm: optical density at 660 nm. Data are expressed as mean ± SD (n = 3).
Fig 4.
Influence of sucrose concentration on the growth of strain WR16-4T.
OD660 nm: optical density at 660 nm. Data are expressed as mean ± SD (n = 3).
Fig 5.
Effect of carbon source combinations on the growth of strain WR16-4T.
F: d-fructose, G: d-glucose, S: sucrose; numbers in square brackets denote % (w/v) of carbon source added. OD660 nm: optical density at 660 nm. Data are expressed as mean ± SD (n = 3).
Fig 6.
Phylogenetic tree of the strain WR16-4T derived from 16S rRNA gene sequence analyses using the maximum-likelihood method.
L. plantarum subsp. plantarum JCM 1149T was used as the outgroup. Numbers at nodes denote the bootstrap values (>90%) for branch points based on 1,000 replications.
Fig 7.
Core genome phylogenetic tree obtained from the concatenated multiple sequence alignments of 81 core bacterial genes using UBCG2.
L. plantarum subsp. plantarum JCM 1149T was used as an outgroup to root the tree. Bipartitions of the phylogenetic tree show the gene support index (GSI) values calculated by UBCG2 of ≥ 95%. The gene support index (GSI) is indicated at branching points in the concatenated phylogenetic tree. The horizontal bar at the base represents 0.10 substitutions per nucleotide site.
Table 2.
Genotypic characteristics of strain WR16-4T and closely related species.
Table 3.
Comparison of the characteristics of strain WR16-4T and closely related species.