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Fig 1.

Genome map of Isopyrum anemonoides mitogenomes.

Different colors represent different genes. The inner genes are transcribed clockwise, and the outer genes are transcribed counterclockwise. The innermost gray pattern represents the GC content.

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Table 1.

General features of mitogenomes from three Ranunculaceae species.

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Fig 2.

Relative synonymous codon usage (RSCU) in the Isopyrum anemonoides mitogenome.

Codon families are shown on the x-axis. The RSCU values represent the frequency of each codon usage relative to the expected frequency based on uniform synonymous codon usage.

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Fig 3.

Repeat analysis of the I. anemonoides mitogenome.

The C1 circle shows the dispersed repeats connected with blue and pink arcs from the center going outward: The C2 circle shows the tandem repeats as short bars. The C3 circle shows the microsatellite sequences identified using MISA.

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Table 2.

Fragments transferred from chloroplast to mitochondria in Isopyrum anemonoides.

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Table 2 Expand

Fig 4.

The GC contents of 3 three Ranunculaceae species mitogenome.

Comparison of GC contents of 33 PCGs between Isopyrum anemonoides, Anemone maxima, and Aconitum kusnezoffii.

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Table 3.

Gene duplication and loss in mitogenomes of the three Ranunculaceae species.

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Table 3 Expand

Fig 5.

The distribution of RNA editing sites in three Ranunculaceae species mitogenome.

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Fig 5 Expand

Fig 6.

dN/dS ratios of 32 PCGs in Isopyrum anemonoides, Anemone maxima, and Aconitum kusnezoffii.

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Fig 7.

Maximum likelihood and Bayesianconsensus tree of I. anemonoides with other 15 plant species.

ML and BI consensus trees were constructed based on the sequences of 25 conserved protein-coding genes using IQ-TREE (ML) and MrBayes (BI). The species and GenBank accession numbers used in the phylogenetic analysis are listed in Additional File 1.

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Fig 7 Expand