Table 1.
List of negative and positive natural CWD cases included in the study.
Fig 1.
Overview about the CWD positive animals and tests used in this study.
Table 2.
Results obtained from the set 1 of samples by the three ELISA rapid tests at NVI.
The O.D. represents the mean of duplicates values obtained from each dilution sample. The values above the cut-off indicate positive sample and those below the cut-off indicate negative sample.
Table 3.
Results obtained from the set 2 of samples analyzed at NVI and IRL using three ELISA rapid tests and two western blot methods.
The O.D. represents the mean of duplicates /triplicates values obtained from each dilution sample by rapid tests. The values above the cut-off indicate positive sample and those below the cut-off indicate negative sample. Pos and Neg indicate positive and negative results by Western blot analyses.
Fig 2.
Confirmatory SAF-immunoblot analysis of proteinase K-treated homogenates on dilution series from brain tissue of positive Moose D.
Lane 1 = undiluted; lane 2 = dilution 1:2; lane 3 = dilution 1:4; lane 4 = dilution 1:8; lane 5 = dilution 1:16; lane 6 = dilution 1:32; lane 7 = dilution 1:64; lane 8 = dilution 1:128. Membranes were probed with mAbs 6H4 (A), Sha31 (B), 9A2 (C), SAF84 (D), L42 (E).
Fig 3.
TeSeE™ western blot analysis of proteinase K-treated homogenates on dilution series from brain tissue of positive Moose D (red rectangles) and positive Moose A, B, C.
Upper panel short exposition, lower panel longer exposition. Membrane A. Lane 1 = molecular weight; lane 2 = positive classical scrapie; 3 = negative moose control; lane 4 to 10: Moose D; lane 4 = undiluted; lane 5 = dilution 1:2; lane 6 = dilution 1:4; lane 7 = dilution 1:8; lane 8 = dilution 1:16; lane 9 = dilution 1:32; lane 10 = dilution 1:64. Membrane B. Lane 1 = molecular weight; 2 = positive classical scrapie; lane 3 = Moose E undiluted; lane 4 = Moose D dilution 1:128; lane 5 to 7: Moose A; lane 5 = undiluted; lane 6 = dilution 1:2; lane 7 = dilution 1:4; lane 8 to 9: Moose B; lane 8 = undiluted; lane 9 = dilution 1:2; lane 10 = Moose C undiluted.