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Fig 1.

Light spectra relevant to intensity based HR assessment.

Typical hemoglobin light absorption spectrum (left) compared to typical optical sensors sensitivity (right).

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Fig 1 Expand

Fig 2.

Camera assemblies at the top of the cage.

Displayed on the left is a PiCam 2 noIR module in its enclosure. The right assembly shows the mount for an FLIR Boson 320 with 50 FOW.

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Fig 2 Expand

Fig 3.

Frontal camera assembly near the water source.

The left assembly contains a PiCam 2 noIR module in its enclosure. The right assembly holds a FLIR Lepton 3 camera module for thermal images.

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Fig 3 Expand

Table 1.

Cameras of the monitoring cage.

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Table 1 Expand

Fig 4.

Home cage designs.

The left design is intended to be used in a cage rack, while the right design is for stand-alone settings.

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Fig 4 Expand

Fig 5.

Illustrated hardware concept.

The hardware consists of a dedicated network router (a), fast SSD data storage (b), two Raspberry Pi 4B computing modules (c) each connected to a Raspberry Pi Camera Module v2 NoIR (d), and a Flir Boson 320 (e) and Flir Lepton v3.5 (f) thermal camera respectively.

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Fig 5 Expand

Fig 6.

User interface.

One-Button design concept, providing an overview of the systems and system sensors status (left) and enabling multi-device access (right).

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Fig 6 Expand

Fig 7.

Example images of cameras.

Captured images of all four integrated cameras, which are segregated into top and frontal views as well as visible light and NIR and thermal views.

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Fig 7 Expand

Fig 8.

Spectral investigation without environmental lighting (top): (1) Inner ear area, (2) Mice’s outer edge, and (3) Mice-free area. The spectral analysis is shown on the right side, and the left side displays the corresponding areas. The spectra indicate notable harmonics at three different ranges: (a) 6 bpm, (b) 100-250 bpm, and (c) 400-600 bpm.

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Fig 8 Expand

Fig 9.

Spectral investigation with environmental lighting (top): (1) Inner ear area, (2) Mice’s outer edge, and (3) Mice-free area. The spectral analysis is shown on the right side, and the left side displays the corresponding areas. The spectra indicate notable harmonics at three different ranges: (a) 6 bpm, (b) 100-250 bpm, and (c) 400-600 bpm.

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Fig 9 Expand

Fig 10.

Spectral investigation without environmental lighting (front): (1) Inner ear area, (2) Mice’s outer edge, and (3) Mice-free area. The spectral analysis is shown on the right side, and the left side displays the corresponding areas. The spectra indicate notable harmonics at three different ranges: (a) 6 bpm, (b) 100-250 bpm, and (c) 400-600 bpm.

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Fig 10 Expand

Fig 11.

Spectral investigation with environmental lighting (front): (1) Inner ear area, (2) Mice’s outer edge, and (3) Mice-free area. The spectral analysis is shown on the right side, and the left side displays the corresponding areas. The spectra indicate notable harmonics at three different ranges: (a) 6 bpm, (b) 100-250 bpm, and (c) 400-600 bpm.

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Fig 11 Expand