Fig 1.
Morphological characteristics of the ten herbal materials of Tiedi Wan.
(A) Ophiopogonis Radix, (B) Platycodonis Radix, (C) Canarii Fructus, (D) Scrophulariae Radix, (E) Fritilariae Thunbergia Bulbus, (F) Trichosanthis Pericarpium, (G) Poria, (H) Glycyrrhiza Radix Et Rhizoma, (I) Membrana Follicularis Ovi, (J) Chebulae Fructus.
Table 1.
GenBank accession numbers of the medicinal materials for making mock samples.
Table 2.
Summary of the data analysis of shotgun meta-sequencing data.
Table 3.
High throughput sequencing (HTS) detection of the DNA barcode sequences of prescribed herbal materials in two TDW samples.
Fig 2.
The number of OTUs and base differences detected in mock samples of Tiedi Wan groups of species.
The size of the bubbles indicates the number of OTUs obtained and the colour of the bubbles indicates base differences. Darker colours indicate more base differences.
Fig 3.
The ITS2 sequences of three commercially available samples were clustered and analysed using MEGAN6.
Each taxonomic the node is drawn as a pie chart indicating the proportion of each species in the taxon for each sample.
Fig 4.
Microscopic identification, the TLC identification, and HPLC chromatogram of pharmaceutical samples.
A. Microscopical identification: a. Irregularly branched mass b. hypha c. Calcium oxalate needle crystal d. stone cell e. amylum body f. Fibrous layer of pericarp g. Connecting tube h. Crystal Sheath Fiber. B. Figure a shows the thin layer chromatogram of gallic acid, 1–3 represent the test solutions of HSZY056, HSZY143 and HSZY144 respectively, and 4 represents the control solution of gallic acid. Figure b shows the thin layer chromatogram of Peimine. 5–7 represent the sample solutions of HSZY056, HSZY143 and HSZY144 respectively, and 8 is the control solution of Peimine. C. HPLC chromatogram of pharmaceutical samples. The peak marked in the figure is the chromatographic peak of glycyrrhizic acid.