Fig 1.
Freshwater microalgae taxa identified across different nutrient media.
Venn diagram illustrating the number of shared and unique freshwater microalgae taxa across different culture media. The number of microalgae taxa identified on media BG-11, BBM, MM, and MS using two identification methods (A) Morphological identification, (B) Molecular-level identification using different DNA barcodes. (C) The total number of microalgae taxa commonly shared or uniquely identified using morphological and molecular identification methods. The genera identified are presented in S5 Table.
Table 1.
List of the algal genera identified in this study through both morphological and molecular- level.
Fig 2.
Venn diagrams illustrating the unique and shared microalgae identified through the different databases (SILVA, GG, and PR2) and the different DNA barcodes sequenced.
Cyanobacteria genera were identified through (A) 16S V1-V3 region and (B) 16S V4-V5 rRNA region. (C) Eukaryotic genera identified through 18S rRNA gene annotated using SILVA database and 16S rRNA gene annotated using PR2 database.
Fig 3.
Relative abundance of cyanobacteria identified through 16S rDNA V1-V3 (top-half) and 16S rDNA V4-V5 region (bottom-half) annotated through (A & D) SILVA, (B & C) GG and (C & F) PR2 databases.
Fig 4.
Eukaryotic microalgae identification.
Relative abundance of eukaryotic microalgae identified through the different databases (SILVA, GG, and PR2) and the different DNA barcodes sequenced. (A-D) are eukaryotic microalgae identified through 18S rRNA gene annotated using SILVA database. Relative abundance of eukaryotic microalgae genera identified using 16S V1-V3 through Greengenes and PR2 (E & F), respectively, 16S V4-V5 rRNA gene (G & H) Greengenes and PR2, respectively.
Fig 5.
Alpha diversity indices of total microbial community based on OTUs.
The total calculated alpha diversity are as follows: Good’s Coverage; Sobs (total number of OTUs observed); Shannon; InvSimpson and Breger-Parker. The first half belonging to the V1-V3 region (A-C) and the second-half represents the V4-V5 region (D-F), and last plot belongs to 18S V4. (A, D) SILVA, (B, E) GG, and (C, F) PR2 database. (G) Alpha diversity indices calculated through 18S rRNA gene and SILVA.
Fig 6.
Non-metric multidimensional scaling analysis (nMDS) computed on Bray-Curtis similarity index obtained for microbial community-identified using SILVA 18S V4, water samples, and media as factors.