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Table 1.

Results from ordination analysis.

CCA of H. valida showing the relationship between venom components matrix (complete and reduced), behavioural and morphophysiological variables assessed across different ecological contexts using two different distances (Chi-square and Bray-Curtis).

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Fig 1.

Canonical correspondence analysis (CCA) of the reduced venom profile matrix.

Used the Bray-Curtis distance (green letters), with the matrix constrained by behavioural and morphophysiological (black letters) variables of the individuals (red letters) of Hadronyche valida.

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Fig 2.

Network plot Hadronyche valida of a regularised canonical correlation analysis (rCCA).

Showing the structure of the association of venom components (complete venom matrix) with behavioural and morphophysiological variables. The correlation cut-off shown is at 0.40. The colour of each line indicates the nature of the correlation (red: Positive association, green: Negative association) between venom components, defence, and heart rate.

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Table 2.

Results from the ordination analysis including H. infensa, H. cerberea and A. robustus.

CCA showing the relationship between the venom components matrix (complete and reduced) and behavioural and morphophysiological variables assessed across different ecological contexts using two different distances (Chi-square and Bray-Curtis).

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Table 2 Expand

Fig 3.

Ordination plot NMDS: Between species comparison.

Separation of individuals of each species based on venom profiles (using Bray-Curtis distance). Red squares = H. valida; green circles = H. infensa; dark blue triangles = H. cerberea, and light blue diamonds = A. robustus. Stress value = 0.148. The lines represent the projection of the significant behavioural (blue: Activity) and morphophysiological (green: Heart rate) variables on the NMSD plot of venom components. Activity decreases as it projects over H. cerberea. In contrast, heart rate increases as it projects over H. cerberea, but shows a more homogenous pattern in the other species.

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