Table 1.
The bioinformatics analysis tools and related websites.
Fig 1.
Agarose gel electrophoresis of cel1 and cel2 from T. aurantiacus cDNA.
M. Trans2K DNA Marker;cel1 and cel2: PCR amplification products with T. aurantiacus cDNA.
Fig 2.
Blast analysis of cel1.
Fig 3.
a. Nickel column affinity chromatography of CEL1. b. Nickel column affinity chromatography of CEL2. c. SDS-PAGE analysis of purified protein. M. marker(10-170kDa); CEL1 and CEL2:Purified products.
Fig 4.
SDS-PAGE analysis of stain protein.
M. marker(10-170kDa); CEL1: stain the target protein.
Fig 5.
SDS-PAGE analysis of purified mutation proteins.
M. marker(10-170kDa); E247A, E267A,E303A,E351A,N68D,N112D,Y150H,CEL1,Y323H,N375D:Purified products.
Fig 6.
The optimum reaction temperature.
Fig 7.
The optimum reaction pH value.
Fig 8.
Effect of metallic ions.
Fig 9.
Effect of organic solvents on enzymatic properties of CEL1 and CEL2.
Fig 10.
Salt tolerance of enzyme.
Fig 11.
Comparison of mutant enzyme activities.
Fig 12.
The 3D structure of CEL1 and CEL2 by computer modeling.
Fig 13.
Missense3D analyzes Glu replaced by Ala.
Fig 14.
Missense3D analyzes amino acid replaced by Asp.
Fig 15.
Missense3D analyzes Tyr replaced by his.