Fig 1.
Whole-genome screening identified modulators of gpdh-1 in dpy-7 worms.
(A) Schematic model of dsRNA screening and scoring of dpy-7(e88);gpdh-1p::dsRed2 worms. (B) Mean plus standard error of relative gpdh-1 mRNA levels in worms fed dsRNA expressing bacteria that changed gpdh-1p::dsRed2 fluorescence. N = 3 to 12 populations of worms, *P < 0.05, **P < 0.01, ***P < 0.001 determined by one-sample t-test with multiple comparisons corrections versus a normalized value of 1.0.
Fig 2.
Effects of gpdh-1 modulators vary with condition.
Mean plus standard error of relative gpdh-1 mRNA levels in worms fed dsRNA expressing bacteria in wild type (A), dpy-7(e88) worms on 51 mM (replotted from Fig 1B) (B), and wild type worms on 200 mM NaCl agar (C). The control RNAi worms in dpy-7 (B) and 200 mM NaCl (C) had gpdh-1 mRNA levels that were elevated 19.1 and 7.9-fold relative to wild type 51 mM controls, respectively. N = 3 to 12 populations of worms, *P < 0.05, **P < 0.01, ***P < 0.001 determined by one-sample t-test with multiple comparisons corrections versus a normalized value of 1.0.
Fig 3.
Glycerol and myo-inositol accumulation pathways interact via feedback.
(A) Mean plus standard error of relative hmit-1.1, hmit-1.2, and hmit-1.3 mRNA levels in dpy-7(e88) worms fed dsRNA for hmit-1.2. N = 4 populations of worms. (B) Mean plus standard error of relative hmit-1.1 mRNA levels in dpy-7(e88) worms fed dsRNA of gpdh-1 modulators. N = 6 to 7 populations of worms, *P < 0.05, **P < 0.01, ***P < 0.001 determined by one-sample t-test with multiple comparisons corrections versus a normalized value of 1.0.
Fig 4.
gpdh-1 modulators affect glycerol accumulation.
Mean plus standard error of whole-worm glycerol levels in wild type (A) and dpy-7(e88) worms (B) fed dsRNA of gpdh-1 modulators. (A and B) N = 3 to 7 populations of worms, *P < 0.05, **P < 0.01, ***P < 0.001 determined by one-way ANOVA with a Dunnett post-hoc test. (C and D) Motility of worms 20 minutes after transfer to agar containing 350 (C) or 550 mM NaCl (D); N = 6 to 10 populations of worms. (A-D) *P < 0.05, **P < 0.01, ***P < 0.001 determined by one-way ANOVA with a Dunnett post-hoc test.
Fig 5.
gpdh-1 modulators are stress response specific.
Mean plus standard error of relative gst-4 (A) and nlp-29 (B) mRNA levels in dpy-7(e88) worms fed dsRNA of gpdh-1 modulators. N = 4 to 8 populations of worms, *P < 0.05, **P < 0.01, ***P < 0.001 determined by one-sample t-test with multiple comparisons corrections versus a normalized value of 1.0.