Fig 1.
Map of sampling sites for B. germanica and P. americana across Iran.
Numbers are 1: Ardabil, 2: West Azarbaijan, 3: Ilam, 4: Khuzestan, 5: Kohgiluye and Boyer-Ahmad, 6: Fars, 7: Kerman, 8: Yazd, 9: Isfahan, 10: Tehran, 11: Mazandaran, 12: Razavi Khorasan, 13: Sistan and Baluchestan. Reprinted from Choubdar et al 2021 under a CC BY license, with permission from PLOS publisher, original copyright. (https://commons.wikimedia.org/wiki/File:Map_of_Iran.png).
Table 1.
Details of cockroach specimens collected in this study.
Fig 2.
Phylogenetic relationship inferred from 443 bp of wsp gene sequences of B. germanica from Iran (OM928504-OM928515 that are shown by black square) and some representative sequences of Wolbachia strains belonging to supergroups A, B, C, D, E, F, G, and M obtained from Genbank database. The Genbank ID numbers, the host species, and the Wolbachia supergroup are shown in the tree branches respectively. The phylogenetic tree was created using Neighbour Joining–Kimura. Scale bar shows genetic distance. Numbers in the tree represent the bootstrap value (bootstrap values below 50% are not shown at the nodes).
Fig 3.
Phylogenetic relationship inferred from 599 bp of gltA gene sequences of B. germanica from Iran (OP146461-OP146466 that are shown by black circles) and some representative sequences of Wolbachia strains belonged to supergroups A, B, C, D, E, F, H, I, J, and K obtained from Genbank database. The Genbank ID numbers, the host species, and the Wolbachia supergroup are shown in the tree branches respectively. The phylogenetic tree was created using Neighbour Joining–Kimura. Scale bar shows genetic distance. Numbers in the tree represent the bootstrap value (bootstrap values below 50% are not shown at the nodes).
Table 2.
Representative wsp and gltA Wolbachia type sequences used in this study.
Table 3.
Prevalence of Wolbachia infection in cockroaches collected from different localities of Iran.