Fig 1.
Heatmap of 31 differentially expressed genes in condyloma acuminata samples.
A numerical version of Fig 1 is presented in S6 Table.
Fig 2.
A–F: Validation using RT-qPCR of immune-related genes that were differentially expressed in condyloma acuminata. The expression of GZMB (A), IFNG (B), IL8 (C), NFATC4 (D), IL12B (E), and IL7 (F) in condyloma samples (HPV6 positive and HPV 11 positive) compared to that in the control (HPV negative samples) is shown as fold change (log2) relative to expression.
Fig 3.
Photomicrographs and graphics showing the cytoplasmatic expression pattern of FOXP3 (A-C) and IFN-γ (D-F). Scale bars: 50 μM in the main pictures and 10 μM in the insets. ANOVA was followed by the Tukey`s Multiple comparison test (± S.E.M. **** = p < 0.0001). Arrows: positive immunostaining for different markers; Ep: stratified squamous epithelium; St: connective tissue stroma of the dermis.
Fig 4.
Photomicrographs and graphics showing the punctual staining pattern of CD1a, corresponding to Langerhans cells (A-C), CD4 (D-F), CD8 (G-I), and CD3 (J-L) expression. Scale bars: 50 μM in the main pictures and 10 μM in the insets. ANOVA was followed by the Tukey`s Multiple comparison test (± S.E.M. * = p < 0.01, ** = p < 0,001 and **** = p < 0.0001). Arrows: positive immunostaining for different markers; Ep: stratified squamous epithelium; St: connective tissue stroma of the dermis.