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Fig 1.

Broiler chickens housed in four environments.

(A) Control, (B) Hay bale, (C) Step platform, and (D) Laser lights.

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Table 1.

Experimental ethogram of recorded broiler chicken behaviors, based on [44].

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Table 1 Expand

Fig 2.

Presence or not of subclinical spondylolisthesis in broiler chickens’ backs at 43 days of age.

(A) Score 0: vertebrae found on their normal axis without compressing the bone marrow, (B) Score 1: vertebrae compressing the bone marrow.

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Fig 2 Expand

Fig 3.

Frequency of observations (mean % ± SEM) of each behavioral category by treatment (C = control, HB = hay bales, SP = step platforms, LL = laser lights) across 2 time periods (09:00 and 15:00 h), n = 192 observations.

Frequencies of (A) resting behavior, (B) exploratory behavior, (C) comfort behavior, and (D) consummatory behavior. Means within behavioral category without a common superscript (a–c) differed at P < 0.05.

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Fig 3 Expand

Fig 4.

Frequency of observations (mean % ± SEM) of each behavioral category by chicken age (in weeks) across two time periods (09:00 and 15:00 h), n = 192 observations.

Frequencies of (A) resting behavior, (B) comfort behavior, (C) exploratory behavior, and (D) consummatory behavior. Means within a behavioral category without a common superscript (a–e) differed at P < 0.05.

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Fig 4 Expand

Fig 5.

Frequency of observations (mean % ± SEM) of behaviors associated with the provided hay bale by age (in weeks) across two time periods (09:00 and 15:00 h).

Sitting or resting by bale (n = 24), Pecking (n = 24), and Resting on top (n = 24). Frequencies without a common superscript (a-c) differed at P < 0.05.

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Fig 5 Expand

Fig 6.

Frequency of observations (mean % ± SEM) of behaviors associated with the provided laser lights by age (in weeks) across two time periods (09:00 and 15:00 h), n = 24.

Frequencies without a common superscript (a-c) differed at P < 0.05.

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Fig 6 Expand

Fig 7.

Frequencies (%) of broiler chickens by gait score (GS) at 21, 28, 35, and 42 days of age, n = 16.

GS 0: Healthy chickens that exhibited no abnormality when walking, GS 1: Chickens that exhibited difficulty walking in a way that was easily identifiable through observation, and GS 2: Chickens exhibiting severe issues walking [45]. Ages without an uncommon superscript (a–d) differed at P < 0.05.

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Fig 7 Expand

Fig 8.

Proportion (%) of broiler chickens with subclinical spondylolisthesis score 1 (vertebrae compressed the bone marrow) at 43 days of age, n = 192.

C = control, HB = hay bales, SP = step platforms, LL = laser lights. Bars without a common superscript (a–c) differed at P < 0.05.

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Fig 8 Expand

Table 2.

Performance (mean ± SEM) of broiler chickens between days 1–21, days 1–35, and days 1–42 of rearing, n = 16.

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Table 2 Expand

Table 3.

Yield (mean % ± SEM) of carcass, breast, wings, legs, back, breast fillet, boneless legs, and abdominal fat of broiler chickens slaughtered at day 43 of age, n = 16.

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Table 3 Expand