Fig 1.
Immunoglobulin profiles from pooled serum samples of dogs with steroid-responsive meningitis-arteritis (SRMA) or intervertebral disc herniation (IVDH) were compared via Discovery Microarray and results were analyzed for common amino acid motifs. Common motifs were compared to amino acid sequences of known proteins and printed on a second customized microarray for further analysis. Abbreviations: SRMA-steroid-responsive meningitis-arteritis; IVDH-intervertebral disc herniation; IL1Ra-interleukin 1 receptor antagonist protein; NNQ-amino acid sequence asparagine-asparagine-glutamine; Ig-immunoglobulin.
Table 1.
In dogs (canis lupus familiaris), 21 proteins were found that contain NNQ data bank search: https://www.uniprot.org/peptide-search/PM20221116faeb6e4d2a474bc2a8a32fca666edb3e/overview.
Fig 2.
Immunoglobulin binding response of dogs with steroid responsive meningitis-arteritis (SRMA) and intervertebral disc herniation (IVDH) in the customized microarray.
Each horizontal line represents 10,000 fluorescence arbitrary units. For a better data overview, the baseline of each sample were shifted up. Abbreviations: Ig-immunoglobulin; IL1Ra-interleukin 1 receptor antagonist protein, CDVN-epitopes from nucleoprotein of canine distemper virus, NNQ-motifs containing the amino acid sequence asparagine-asparagine-glutamine.
Fig 3.
Protein structure of canine Interleukin 1 receptor antagonist protein (IL1Ra) (modified after https://alphafold.ebi.ac.uk/entry/Q9BEH0 [34]) with IgG and IgA binding epitopes.
Abbreviations: SRMA-steroid-responsive meningitis-arteritis; IVDH-intervertebral disc herniation; Ig-immunoglobulin; EEAMMVTKFYFQKE, RPCRMQAFRIWDVN, DVNQKTFYLRNNQ—amino acid sequences [28].