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Fig 1.

Labyrinth spider (Agelena labyrinthica) sitting in her web in the field with a captured honey bee prey (Apis mellifera).

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Fig 2.

QR code to access a video showing a labyrinth spider (Agelena labyrinthica) capturing an adult honey bee (Apis mellifera) in the feeding experiment (URL: https://youtu.be/kO5B2Z7OiNw).

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Table 1.

Primers used for the relative virus quantification of viruses in spiders, Agelena labyrinthica.

Primers used for sequencing of PCR products are marked with asterisk (*).

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Table 2.

Detection of viruses known from honey bees (Apis mellifera) in field-collected labyrinth spiders (Agelena Labyrinthica; N = 16).

Samples tested positive are marked with [+] and negative ones with [–]. The number of samples indicates how many individually analysed spider samples matched each virus profile.

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Fig 3.

Titres of (A,C) Acute bee paralysis virus (ABPV) and (B,D) Deformed wing virus type B (DWV-B) in labyrinth spiders (Agelena labyrinthica) from the experimental feeding trial: (A & B) Virus titres for the two treatment groups that differed with regard to the feeding regime (controls = no virus, treatment = virus); (C & D) female spiders that either did or did not build a cocoon. Boxplots are displayed with the inter-quartile-ranges (box), medians (black line in box) and outliers (dots). Dashed lines represent virus detection thresholds. Significant differences (p < 0.05) between the groups are indicated by small bold letters (a, b).

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Table 3.

Titres of ABPV and DWV-B reported as medians [1st 3rd quartiles] in labyrinth spiders (Agelena Labyrinthica; N = 24) after an experimental feeding assay with infected honey bee pupae (Apis mellifera).

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