Table 1.
Gene expression data from Gene Expression Omnibus (GEO) database.
Fig 1.
Identification of molecular subtypes in PCOS based on preterm labor or labor-related genes.
(A) Chromosomal distribution diagram of preterm labor or labor-related genes involved in the present study. (B) The relationship among preterm labor or labor-related genes of interest were shown. (C) The cumulative distribution function (CDF) curves of consensus scores based on different subtype number (k = 2, 3, 4, 5, 6) and the corresponding color are represented. (D) The CDF Delta area curve of all samples when k = 2. (E) A relative stable partitioning of the samples at k = 2 in consensus heatmap. (F) In PCA analysis, the symbols represent the gene expressed differently in two clusters. (G) The expression heatmap of the preterm labor or labor-related genes among various molecular and clinical subtypes.
Fig 2.
Construction of expression modules by WGCNA package.
(A) Analysis of the scale-free fit index for various soft-thresholding powers and analysis of the mean connectivity for various soft-thresholding powers. (B) The cluster dendrogram of genes. Each branch represents one gene, and every color below represents one expression module. (C) Heatmap of the correlation between module genes and the clusters. (D-F) Top 10 terms from a GO analysis of molecular function, biological process and cellular component in the blue and turquoise modules. (G) Top 10 terms were clustered by KEGG pathway analysis in the blue and turquoise modules.
Fig 3.
Grouping based on hub genes of PCOS.
(A) Top10 hub genes were identified and PPI networks were established with Cytoscape. (B) Venn diagram for the overlapping genes between the above 10 hub genes and hub genes for the key modules. (C) The expression comparison of the five overlapped hub genes between cluster1 and cluster2. (D) The relationship among these overlapped hub genes were shown. (E) A relative stable partitioning of the samples at k = 2 in consensus heatmap. (F) In PCA analysis, the symbols represent the gene expressed differently in hub_cluster1 and hub_cluster2. (G) The expression heatmap of these overlapped hub genes in hub_cluster1 and hub_cluster2. (H) The expression comparison of the five overlapped hub genes between hub_cluster1 and hub_cluster2. (I) Sankey diagram for the links among the preterm labor or labor related gene clusters, hub gene clusters, age subgroups and adipose tissue subtypes of PCOS.