Fig 1.
Maize chlorotic mottle virus (MCMV) distribution in different dissected maize seed tissues.
Maize seed from MCMV infested seed lots were dissected into embryo, endosperm, pedicel, and pericarp tissues, then either processed directly for ELISA (Non-washed) or the whole kernel (External wash) or excised embryo (Embryo wash) was washed in 0.01% Triton X-100 containing 15% bleach for 15 min. prior to processing for ELISA. ELISA absorbance (405 nm) normalized to their respective negative controls are shown. Means for treatment*tissue with the same letter below the bar are not significantly different (p<0.05).
Table 1.
Maize chlorotic mottle virus transmission from infested seed lots collected from Hawaii and Kenya.
Table 2.
Detection of maize chlorotic mottle virus (MCMV) in seed tissues by immunocytochemical microscopy.
Fig 2.
Localization of maize chlorotic mottle virus (MCMV) in seed tissues sections.
Representative images of the distribution of MCMV in maize kernel pericarp (A—C) and pedicel (D—F) tissues as determined by fluorescent immunolocalizations using MCMV specific antibody followed by Alexa fluor 488 secondary antibody (green).
Table 3.
Transmissibility of maize chlorotic mottle virus (MCMV) from seed by vascular puncture inoculation.