Fig 1.
Shotgun metagenomic sequencing results.
Mean relative abundance of the leopard cat diet-related phyla detected from their fecal samples are shown (n = 11). The reads classified into the family Felidae were excluded because they may be derived from DNA of the leopard cat itself. (A) Relative abundance of the three leopard cat diet-related phyla in each sample. Mean relative abundance of each class within each phylum of (B) Chordata, (C) Streptophyta, and (D) Arthropoda.
Fig 2.
Vertebrate mitochondrial 12S rRNA gene sequencing results.
Relative abundance of the top 20 genera identified from fecal samples (n = 22) by vertebrate mitochondrial 12S rRNA gene sequencing is shown. The ambiguous reads that were not identified at the genus level and the reads that were assigned to genera belonging to the family Felidae were excluded from the calculation. The dendrograms represent the similarity (dissimilarity) of log-transformed relative abundance results between samples (x-axis) or between prey animals (y-axis). The dissimilarity is represented by branch lengths based on Euclidean distance.. Abbreviation: CN, Chungcheongnam-do; GB, Gyeongsanbuk-do.
Fig 3.
Comparisons between shotgun metagenomic and vertebrate mitochondrial 12S rRNA gene sequencing.
The class-level results are shown. The reads assigned to the family Felidae were excluded from the comparisons. (A) Relative abundance of classes within the phylum Chordata. (B) Alpha diversity. The Chao1 estimator (community richness) and Shannon index (community diversity) based on the class-level results are shown. (C) Beta diversity. Non-metric multidimensional scaling (NMDS) plots based on the Bray–Curtis dissimilarity (community structure) and Jaccard index (community membership) based on the class-level results are shown.
Table 1.
Major genera detected and their inhabitation status in Korea.
Twenty most abundant vertebrate and chordate genera detected by vertebrate mitochondrial 12S rRNA gene (n = 11) and/or shotgun metagenomic (n = 11) sequencing are shown.