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Fig 1.

Fully metamorphosed Solea solea larva.

A fully metamorphosed larva showing full asymmetrical body, rounded head and complete left eye migration.

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Table 1.

Main differences between the pilots and the experiment conducted.

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Table 1 Expand

Fig 2.

Experimental design.

Number of plates used per treatment at both experiment 1 and 2 (a) and timeline protocol followed during experiment 2, for larvae reared at 16°C (control temperature treatment) and at 20°C (RCP 8.5 temperature treatment), that included a temperature acclimation for larvae.

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Fig 2 Expand

Table 2.

Lethal time for 50% (LT50, days) Solea solea larvae reared at two temperatures with different feeding frequencies and total survival at the end of the experiment.

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Table 2 Expand

Fig 3.

Larvae survival during the experiment.

Percentage of survival with fitted four parameter logistic regressions in S. solea according to the feeding frequency at two different temperatures. a: whole experiment duration (33 days larvae aged 35 dph). b: Solid lines represent linear models fitted to the to the second’s week percentage of survival with 95% confidence level intervals (shadow area).

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Fig 3 Expand

Fig 4.

Length of sole (mm) larvae reared at different temperatures and different feeding frequencies in experiment 2.

Left panels: larvae reared at 16°C. Right panels: larvae reared at 20°C at high (blue), medium (yellow) and low (grey) feeding frequencies.

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Fig 4 Expand

Table 3.

Summary of biometric data at the end of the experiment (35 dph).

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Table 3 Expand

Fig 5.

Dry weight of sole (mm) larvae reared at different temperatures and different feeding frequencies in experiment 2.

Left panels: larvae reared at 16°C. Right panels: larvae reared at 20°C at high (blue), medium (yellow) and low (grey) feeding frequencies.

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Fig 5 Expand

Fig 6.

Metamorphic index in Solea solea larvae.

Differences in the metamorphosis onset and development of larvae reared at two temperatures and 3 different feeding frequencies (high, medium and low food).

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Fig 6 Expand