Table 1.
Overview on bacterial strains and isolates used in this study and the culture media used.
Table 2.
Overview of versions and characteristics of analysis pipelines and 16S reference databases used in this study.
Table 3.
Correctly identified isolates on species or genus level using five different analysis pipelines.
Fig 1.
Fractions of correctly assigned sequences on the species- and genus-level after sequencing of V1-2 and V3-4 16S rRNA gene regions and sequence analysis using different pipelines and 16S rRNA reference databases.
SG = SmartGene IDNS pipeline, LTP = All Species Living Tree database, GTDB = Geome Taxonomy Database, GG = Greengenes Database, SILVA = SILVA database.
Table 4.
Re-assessment of pipeline performances for species assignment based on achievable resolutions within the 16S rRNA gene.