Fig 1.
Organizational chart for RT-PCR testing in shelter dogs.
2PEL = 2 positive eventually lost; 2PEN = 2 positive eventually negative. Created with BioRender.com.
Fig 2.
Duration of excretion of CDV RNA over time in 2PEL and 2PEN dogs.
2PEL (n = 100) indicates those dogs that had at least two positive results and were eventually lost to follow-up. 2PEN (n = 325) indicates those dogs that had at least two positive results and eventually tested negative. Samples were collected by swabbing the inside of one or both nares and/or the deep pharyngeal region.
Table 1.
Comparison of RT-PCR and virus isolation of commercially acquired and field isolates of CDV.
Fig 3.
RT-PCR cycle threshold (Ct) over time(days) in six dogs.
Graph (a) represents dog 1, (b) represents dog 2, (c) represents dog 3, (d) represents dog 4, (e) represents dog 5, and (f) represents dog 6. Red filled circles indicate samples where virus isolation was also positive. Black filled circles indicate samples where virus isolation was negative. Grey circles indicate samples where only RT-PCR testing was done. The green line demarcates the 40 cycle limit of detection. ** Indicates sample where the vial was broken and may have affected virus isolation results.
Table 2.
Virus isolation results and Ct of samples taken over time for dogs 1–6.