Fig 1.
Dorsal section of Xenopus laevis and assignment to BCS 1–5, red line in BCS1 indicates the nose-cloaca distance.
Fig 2.
Lateral section of Xenopus laevis and assignment to BCS 1–5.
Fig 3.
Close-ups pictures of BCS 1: A: dorsal view, B and C: ventral and latero-ventral view, D: lateral view. Humane endpoint: Frog emaciated (—): rectangular body shape; raised abdominal contour and flat dorsal contour; bony structures of the thorax as well as pelvis prominent in ventral view; lower side of the mouth with concave curvature (sunken); skinny limbs firmly set off from trunk; humane endpoint reached.
Table 1.
Five classes of body condition score assigned by body weight (g).
Fig 4.
A. Violin plot of variance distribution in each BCS class. The violin plot shows the compact distribution of data in each BCS class. Individual data points are jittered within each category. The mean body weight values are also offered as red dots, and the error bars represent 1000-fold bootstrapped 95% confidence intervals. B. Scatterplot showing the body weight vs. the nose-cloaca distance color-highlighted by BCS classes. The linear trend in the data (βbw = 0.02, SE = 0.002, p<0.001, R2adj = 0.665) is indicated by the linear regression line with a 95% confidence band.
Table 2.
Linear regression model of BCS ~body weight.
Table 3.
Linear regression model of nose-cloaca length ~bw + bw:BCS.
Fig 5.
High-grade lymph sac edema prior (A) and post puncture (B) of the same specimen, (C) drained clear punctate in syringes. Difference between BCS 5 and pathological lymphatic sac edema: Lymphatic sac edema is indicated by an apparent retraction of the skin between the eyes (see blue marks upper row); skin on the back and thighs shows wave-shaped movements when the frog in question swims due to the edema fluid; the frog swims more sluggishly; no impairment of food intake; clear secretion is drawn off during puncture, and lymph sac collapses again immediately (see blue arrows lower row).