Fig 1.
Foals born after a SCNT procedure using male-horse-derived MSCs as nuclear donors.
Male: left, female: right.
Table 1.
In vitro and in vivo development of embryos obtained after a SCNT procedure using MSCs of a male donor.
Fig 2.
Microsatellite profiles from the foals.
Results from the analysis of a 15-panel of microsatellites, used for routine parentage and identity purposes.
Fig 3.
Representative metaphase spreads from the foals and the donor MSCs.
(A) G banding and (B) C banding from the MSCs used as nuclear donors (64,XY). (C) C banding from the male clone (64,XY). (D) C banding from the female clone (63,X). Arrows indicate the Y chromosome and arrowheads indicate the X chromosome. Magnification 1000X.
Fig 4.
Specific identification of the X and Y chromosomes by FISH.
(A) and (B) Blood cells from the female foal. (C) Metaphase spread derived from the blood sample of the male foal. (D) Interphase cells from the MSC culture used as nuclear donor. Red color indicates the X chromosome probe and green color indicates the Y chromosome probe. Magnification 1000X.
Fig 5.
Characterization of X- and Y-linked markers.
Results of microsatellite analysis in the female and male horses. Boxes indicate the Y-linked markers.