Table 1.
The list of specimens from human term placenta for NAEs analysis.
Fig 1.
The concentrations of N-acylethanolamines in various placental tissues and fluids.
Concentrations of palmitoylethanolamide (PEA) (A), oleoylethanolamide (OEA) (B), and anandamide (AEA) (C) are expressed in ng/g, except for umbilical serum (US)—in ng/ml. The average concentrations of PEA, OEA, and AEA values obtained in AM and PL samples independently of their sub-area location are also presented (AM-mean, PL-mean). P-value: P < 0.05*; P < 0.01**; P < 0.001***; ns = non-significant; AM = amniotic membrane, ACM = amniochorionic membrane, PL = placental tissue, UC = umbilical cord, VX = vernix, d = decontaminated tissue.
Fig 2.
Concentrations of palmitoylethanolamide (PEA), oleoylethanolamide (OEA), and anandamide (AEA) in AM samples cultured in cell culture medium (DMEM) or physiological saline (PS).
The samples were incubated at 4°C, 25°C, 37°C for 24 hours.