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Fig 1.

The TLR4-2-Bromoergocryptine Mesylatemesylate complex 3D structure, as well as the 3D and 2D interaction diagram.

The left panel shows a 2D interaction plot of 2-Bromoergocryptine Mesylate and TLR4. The Pi-Alkyl bond is shown by pink dashed lines, whereas residues wrapped inside a light green sphere are thought to be involved in Van der Waals interactions. The binding cavity of the 2-Bromoergocryptine Mesylate molecule is shown in the center panel of TLR4, while the right panel shows a zoomed-out binding pocket with amino acid residues surrounding the molecule.

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Table 1.

10 ligands with the most auspicious binding affinity with TLR4 was calculated by molecular docking analysis.

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Table 1 Expand

Fig 2.

MD simulation trajectory analysis of Root Mean Square Divisions (RMSD) of Bromocriptinemesylate bound with TLR4 at 150 ns time frame.

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Fig 2 Expand

Fig 3.

MD simulation trajectory analysis of hydrogen bonding of 2-Bromoergocryptine Mesylatebound with TLR4 at 150 ns time frame.

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Fig 4.

MD simulation trajectory investigation of 2-Bromoergocryptine Mesylateassociated with TLR4 RMSF at 150 ns time frame shown.

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Fig 5.

MD simulation trajectory analysis of Radius of Gyration (RoG) of 2-Bromoergocryptine Mesylatebound with TLR4 at 150 ns time frame displayed.

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Fig 6.

Protein-ligand contact histogram (H-bonds, hydrophobic, ionic, water bridges) of TLR4 and 2-Bromoergocryptine Mesylate.

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Fig 7.

Secondary structure element distribution by residue index throughout the protein structure.

Red indicates alpha helices, and blue indicate beta-strands of TLR4 and 2-Bromoergocryptine Mesylate.

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Fig 7 Expand