Fig 1.
Nectarine trees showing fruit tree decline syndrome (FTDS) in southern Ontario, Canada.
Table 1.
Strains used in the phylogenetic analysis with their culture accession and GenBank numbers.
Strains from this study are in bold and ex-types are marked with*. NA: not available.
Table 2.
Genomes used in phylogenomic analyses.
Strains from this study are in bold. NA: not available.
Fig 2.
Maximum likelihood (ML) phylogeny based on a concatenated ITS, LSU, act, tef1-α sequence alignment.
Bootstrap support values for ML ≥ 50% and BP ≥ 0.90 are defined as ML/BP above and below the nodes. Strains of the new species are in bold, and ex-type strains are marked with*. The tree is rooted to Diaporthe vaccinii (CBS 160.32).
Fig 3.
C. paraplurivora on P. persica var. nucipersica (FDS-564).
A. Habit of conidiomata on branches. B. Longitudinal section through conidiomata. C. Transverse section of conidiomata. D. Conidiogenous cells. E. Conidia. F. Seven-day-old culture on PDA. Scale bars: A = 200 μm B-C = 100 μm, D-E = 5 μm.
Fig 4.
A. ML tree indicating the placement of C. paraplurivora FDS-564 among 17 species of Ascomycetes. Bootstrap support for all nodes is 100. B. Dot plot graph showing syntenic blocks between genome sequences of C. leucostoma SXYLt and C. paraplurivora FDS-564.
Fig 5.
A. Mean lesion length caused by C. paraplurivora in Prunus spp. 12 days post-inoculation. B-D. Symptoms caused by C. paraplurivora (FDS-564) in excised branches of P. persica var. nucipercica 12 days post-inoculation.
Fig 6.
Mean lesion length caused by C. paraplurivora isolates FDS-564 and FDS-623 in Prunus persica var. persica seedlings 7, 10, 13 and 27 days post-inoculation.
Fig 7.
Symptoms of Cytospora paraplurivora infection on Prunus persica var. persica seedlings including A) 5-day old canker (ck) and gummosis (g); B) 7-day old canker; and C) 27-day old canker, conidiomata (cd) and gummosis.