Fig 1.
The endogenous NLRP6 expression is not detected in HCT116 and RKO cancer cell lines by western-blotting using an antibody from ABGENT.
Western-blot analysis of extracts of HCT116 and RKO cells made 48 or 72hrs after transfection with a pCDNA3.1+ vector either empty or encoding the human full-length FLAG-NLRP6 protein. The migration position of NLRP6 is indicated with a dark arrowhead. The white arrowhead points a non-specific band at 90kDa.
Fig 2.
The endogenous NLRP6 expression is barely detected in several human and mouse cell types by western-blotting using an antibody from ABGENT.
(A) Western-blot analysis of extracts of HCT116 cells transiently transfected by the FLAG-NLRP6-expressing pCDNA3.1+ vector, and of a series of various non-transfected cell lines. The upper image corresponds to a short exposure and the lower image to a longer exposure. (B) Western-blot analysis of the same extracts as in A and total mouse colon extracts. The migration position of NLRP6 is indicated with a dark arrowhead. The white arrowhead points a non-specific band et 9OkDa. The amount of loaded total proteins is indicated for each lane.
Fig 3.
The endogenous NLRP6 protein is present only in cytoplasmic dots also containing the ASC protein.
(A) Immunofluorescence microscopy images of HCT116 cells showing the colocalization of NLRP6 and ASC in small cytoplasmic dots (white arrows). (B) Immunoprecipitation of NLRP6 in HCT116 and RKO cell extracts followed by an analysis with anti-NLRP6 antibody from ABGENT. NLRP6 is indicated with a dark arrowhead. The white arrowhead points a non-specific doublet at 90kDa.