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Fig 1.

Image of leaf disc locations.

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Fig 2.

Heatmap representation of Ct values from duplicate RT-qPCR assays of each disc for a specific leaf.

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Fig 3.

Relationship between number of leaves in a mixed batch of leaves and increase in Ct where batches comprise a single ASBVd-infected leaf pooled with healthy avocado leaves.

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Table 1.

Index of notation for parameter values, with default values, and variables used in computing an optimal sampling design for disease-free status.

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Table 2.

Optimal sample sizes for a selection of inputs.

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Table 3.

Optimal sample sizes for different cost functions.

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