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Fig 1.

Flow of participants.

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Fig 2.

Experimental approach.

All participants will undergo two testing conditions. In randomized order, in prone position, the ankle will be moved between plantar flexion and dorsal extension by use of a dynamometer (arrows) with the knee in extended (A) and flexed (B) position. In both conditions, ultrasound recordings of the calf and dorsal thigh will be made to estimate tissue displacement induced by the ankle motion.

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Fig 3.

Exemplary visualization of US cross-correlation analysis.

Here, five ROIs (red rectangles) have been positioned at rest (left image): three within the fascia and one in each, the subcutaneous tissue (SCT) and the muscle ROIs. The small yellow squares within the ROIs represent the center of the non-moving ROIs. Upon movement (right image), pixel displacement relative to the center of the non-moving ROIs are tracked (yellow line in right picture). The end of the line indicates the maximal displacement, computed by the software algorithm.

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