Fig 1.
Number of stomata in diploid and triploid tea leaves.
A: Number of diploid stomata and triploid stomata at 100X magnification; B: Number of diploid stomata and triploid stomata at 1000X magnification; C: Length and width of diploid and triploid stomata; D: Diploid and triploid stomatal density in the same field of view. Error bars indicate SD (n = 3); statistical significance is indicated *P<0.05, **P<0.01.
Fig 2.
Transverse section of tea diploid and triploid leaf veins.
A: Diploid and triploid vein xylem cross section under 50X magnification. B&C: Statistical data for cross sections of diploid and triploid leaf vein xylem blades. Error bars indicate SD (n = 3); ** indicates significant difference at the 0.01 level between the diploid and triploid.
Fig 3.
Transverse section of tea diploid and triploid leaf mesophyll.
A: Diploid and triploid mesophyll cross sections under 100X magnification. B-E: Cross sectional length of upper epidermal cell, size of the palisade tissue cell, number of sponge tissue cells and sponge tissue length. Error bars indicate SD (n = 3); ** indicates significant difference at the 0.01 level between the diploid and triploid.
Fig 4.
Unigene database statistics, functional annotations and Venn diagram.
A: The X-axis represents the database, where All represents the union of all the data; the Y-axis represents the number of unigenes in the corresponding database annotation, and the number in the figure corresponds to the number of unigenes in the annotation. B: Venn diagram showing the number of specific genes between the two samples.
Fig 5.
The abscissa is the GO term for the next level of the three major categories in GO, and the ordinate is the number of genes annotated to a term (including the subterm of the term). The three different categories represent the three basic tree classifications for GO terms (from left to right, biological processes, cellular components, molecular functions).
Fig 6.
COG function classifications of unigenes for Clari as macrocephalus.
Left-axis indicates the number of unigenes; letters on the x-axis represent different COG categories.
Fig 7.
Expression patterns of genes related to growth and development of diploid and triploid tea leaves analyzed by qRT-PCR.
Fig 8.
Pathway enrichment analysis of differentially expressed genes.
Fig 9.
Forty DEGs (red) involved in the photosynthesis pathway were up-regulated in QianMei 419 compared to QianFu 4.
DEGs (green) involved in the photosynthesis pathway were down-regulated in QianMei 419 compared to QianFu 4. Red and green boxes indicate genes whose expression levels were up and downregulated between QianMei 419 and QianFu 4. For interpretation of the references for the color in this figure legend, the reader is referred to the web version of this article.
Table 1.
Differentially expressed genes involved in photosynthesis pathway.
Table 2.
Differentially expressed genes (DEGs) related to stomatal development in diploid and triploid.
Table 3.
Differentially expressed genes involved in leaf developmental growth in diploid and triploid.
Table 4.
Transcription factor identified in the DEGs involved in leaf developmental in diploid and triploid.