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Fig 1.

Photograph of the pilot-scale AST system.

Right/rear: Primary clarifier; middle: aeration basin; left/front: secondary clarifier.

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Fig 1 Expand

Fig 2.

Schematic diagram of the pilot-scale AST system.

Note that sample port 2 (SP2) is not used for this study.

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Table 1.

Experimental conditions for all experiments.

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Table 1 Expand

Fig 3.

globigii concentration response curves for Test 1 and 2.

B. Concentration-time plot of individual sample ports over time for experiment 1 and experiment 2.

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Fig 3 Expand

Fig 4.

Averaged B. globigii concentrations and TSS in the RAS and aeration basin effluent.

The light gray block shows the possible HRT range of 0.375 to 3.375 days, a black vertical line is the average calculated SRT of 23.6±5.6 days, and the darker gray block represents the possible SRT range of 18–32.1 days. SP1 and SP3 from both experiments were averaged for “average primary clarifier,” and likewise for SP4 and SP5 for “Average aeration basin.” The “+” and “x” series show the TSS in the aeration basin effluent and RAS, respectfully. Error bars were removed for visual clarity. Original data in S2 Text.

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Fig 4 Expand

Fig 5.

MS2 concentration response curves for Test 1 and 2.

Concentration-time plot of individual sample ports over time for experiment 1 and experiment 2.

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Fig 5 Expand

Fig 6.

Averaged MS2 concentrations and TSS in the RAS and aeration basin effluent.

De-Cl = “dechlorinated water” and SWW = “synthetic wastewater.” The light gray block shows the possible HRT range of 0.375 to 3.375 days. The black vertical line is the average calculated SRT of 15.0±10.9 days and the darker gray bar represents the SRT possible range of 4.1–28.9 days, respectively. SP1 and SP3 were averaged for “average primary clarifier” series. SP4 and SP5 were averaged for “average aeration basin” series. The “+” and “x” series show the TSS in the aeration basin effluent and RAS, respectfully. Error bars were removed for visual clarity. Original data in S2 Text.

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Fig 6 Expand

Fig 7.

Phi-6 concentration response curves for Tests 1 and 2.

Concentration-time plot of individual sample ports over time for experiment 1 and experiment 2.

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Fig 7 Expand

Fig 8.

Averaged MS2 concentrations and TSS in the RAS and aeration basin effluent.

The light gray block shows the HRT range of 0.75 to 1.75 days. The black vertical line is the average calculated SRT of 23±2 days and the darker gray bar represents the SRT possible range of 21–26.4 days. SP1 and SP3 were averaged for “Average primary clarifier.” SP4 and SP5 were averaged for “Average aeration basin.” The “+” and “x” series show the TSS in the aeration basin effluent and RAS, respectfully. Error bars removed for clarity (Original data in S2 Text).

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Fig 8 Expand