Fig 1.
Real-time quaking-induced conversion (RT-QuIC) analysis of ear and belly skin tissue sampled from CWD-positive white-tailed deer.
Box plots indicate the median with a horizontal red line, second and third quartiles with the box, and first and fourth quartiles with the whiskers. Statistical outliers are indicated by red crosses. Controls are known CWD-positive and negative obex run at 10−3 dilutions. The horizontal black line at 40 h indicates the end time of the assay. (A) Ear time to threshold results from samples at dilutions of 10−2. (B) Belly skin results from tissue samples at 10−2 dilutions.
Fig 2.
Ear sampling locations on white-tailed deer pinnae.
Seven easily accessible locations in ear pinnae were tested for sensitivity and specificity on whole ears from seven CWD-positive deer. Deer tagging is usually performed on one or both ears and is primarily placed near location 7.
Fig 3.
Time-to-threshold measurements of seven ear sample sites and obex of seven CWD-positive white-tailed deer.
Box plots indicate the median with a horizontal red line, second and third quartiles with the box, and first and fourth quartiles with the whiskers. Statistical outliers are indicated by red crosses. The horizontal black line at 40 h indicates the end time of the assay. B is a 10−3 dilution of obex tissue from the indicated deer, and 1 through 7 indicate the sampling locations (see Fig 2) used to prepare 10−2 dilutions of ear skin tissue. The control deer is not shown.
Table 1.
Assay results from white-tailed deer.
Table 2.
Disagreement between postmortem immunohistochemistry (IHC) and real-time quaking-induced conversion (RT-QuIC) results.