Fig 1.
Comparison of the visceral sensitivity of rats in different groups.
A: Pressure thresholds leading to the arching of back; B: Pressure thresholds leading to the raising of abdomen.**P<0.01.
Table 1.
The number of DE proteins between different groups.
Fig 2.
Comparisons of the number and overlapping relationships of differentially expressed proteins between different samples.
Yellow circle and purple circle represents number of DEPs in different comparable groups. A: comparable groups: T vs M and M vs C; B: comparable groups: SD vs M and M vs C; C: comparable groups: SD vs M and T vs M.
Table 2.
Important shared DE proteins in two comparing groups.
Table 3.
Protein changes unique to group T and group SD relative to group M.
Fig 3.
Comparing functional annotations of DEPs between group M and group C.
The green bars represent biological process; orange bars represent cellular component; purple bars represent molecular function.
Fig 4.
Comparing functional annotations of DEPs between group T and group M.
The green bars represent biological process; orange bars represent cellular component; purple bars represent molecular function.
Fig 5.
Comparing functional annotations of DEPs between group SD and group M.
The green bars represent biological process; orange bars represent cellular component; purple bars represent molecular function.
Fig 6.
Comparing functional annotations of shared DEPs in comparable groups group T vs group M and group SD vs group M.
The green bars represent biological process; orange bars represent cellular component; purple bars represent molecular function.
Fig 7.
Cluster analysis of differentially expressed proteins and visualization of the changing proteins and their KEGG biological pathways.
A: Comparable group: group M and group C; B: Comparable group: group T and group M; C: Comparable group: group SD and group M.
Fig 8.
Protein-protein functional network analysis of DEPs between group M and group C.
A: The protein-protein functional network. The network was constructed using STRING database and Cytoscape software. B: The top 10 key proteins in the protein-protein interaction networks.
Fig 9.
Protein-protein functional network analysis of DEPs between group T and group M.
A: The protein-protein functional network. The network was constructed using STRING database and Cytoscape software. B: The top 10 key proteins in the protein-protein interaction networks.
Fig 10.
Protein-protein functional network analysis of DEPs between group SD and group M.
A: The protein-protein functional network. The network was constructed using STRING database and Cytoscape software. B: The top 10 key proteins in the protein-protein interaction networks.
Table 4.
Important MCL clusters of DEPs.
Fig 11.
ATP5a1 and BPNT1 expressions in colonic mucosa.
The protein level of ATP5a1 and BPNT1 were analyzed by western blot. A. Quantitative analysis of ATP5a1 and BPNT1 expression in colonic mucosa (each group, n = 4, ANOVA). B. All values were expressed as the mean ± SD, **P<0.01: compared with Group C; ##P<0.01: compared with Group M; ns: no significant. Bonferroni’s post hoc test was used for multiple comparisons.