Table 1.
PCR primers used in phage isolation.
Fig 1.
PCR product of raw water samples from MN-WWTP.
(A) Lane 1: DNA ladder (100–2000 bp), Lane 2: negative control, Lane 3,4: negative samples, Lane 5: 476 bp (Myoviridae, DGF primer), Lane 6: 459 bp (Siphoviridae, MCF-2 primer), Lane 7: 500 bp (Myoviridae, CTF primer) and Lane 8: 774 bp (Podoviridae, MCF-3 primer).
Fig 2.
PCR product of raw water samples from KSU-WWTP.
Lane 1: DNA ladder, Lane 2: negative control, Lane 3–6 and 8: negative samples, Lane 7: 500 bp (Myoviridae, CTF primer).
Fig 3.
Prevalence of phage families in WWTPs.
Fig 4.
Temporal prevalence of phage families in WWTPs during four-month period.
Table 2.
The significance of the influence of temperature on prevalence of phage families in wastewater treatment plants.
Fig 5.
Frequency of phage families across different temperature ranges in WWTP.
Table 3.
The significance of the influence of temperature on prevalence of phage families in wastewater treatment plants.
Fig 6.
Relative abundance of phage sequence in NGS-defined sewage samples.
M1: first sample from MN-WWTP, M8: eighth sample obtained from MN-WWTP, K1: first sample obtained from KSU-WWTP and K5: fifth sample obtained from KSU-WWTP.
Table 4.
Pearson’s correlation matrix of different phage species in positive samples.
Fig 7.
Occurrence of bacteriophages in NGS samples in WWTPs.
Fig 8.
Occurrence of bacteriophages monthly in NGS samples.