Table 1.
Composition and calculated contents of the experimental diets.
Table 2.
Nucleotide sequences of the primers used for quantitative real-time RT-PCR.
Table 3.
Growth performance.
Table 4.
Body composition of broiler chick at 1 day and 42 days of age.
Fig 1.
Representative reconstructed 2D images of market-age-broiler femur metaphysis (42 days of age).
(A) reconstructed images of cortical porosity (purple) within the cortical bone; (B) lateral view of the reconstructed cortical porosity extracted from bone, indicated the cortical bone pore size, pore volume and porosity ratio.
Table 5.
Femur metaphysis section 3D structure data at market-age broilers.
Fig 2.
Reconstructed 2D structure of tibia diaphysis on day 18 embryo.
Haversian artery was used as a landmark for region of interest (ROI) selection. The diaphysis bone traits were scanned and analyzed by μCT.
Table 6.
Metaphysis microstructure of tibia on day 18 embryo.
Fig 3.
The relative mRNA expression of adipogenesis and osteogenesis marker genes.
Maternal fish oil supplementation suppressed bone marrow adipogenesis but didn’t change osteogenesis in offspring broiler. Expression of A) adipogenesis marker genes; B) osteogenesis marker genes; The relative mRNA expressions were detected by qPCR method in SO and FO group. Total RNA from femur bone marrow from 42 day-old-broiler were collect and reversed (N = 6). Fold changes of gene expression were calculated using the ΔΔCt method by student’s t-test. Each experiment was repeated at least 3 times. The error bars represent SEM. * means there was a statistically significant difference between treatments, p < 0.05.