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Table 1.

Physicochemical properties of the three agricultural soils (0–20 cm soil layers).

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Fig 1.

Dynamic changes of NH4+-N and NO3−-N of different treatments in three soils.

Treatment: Control, no fertilizer and nitrification inhibitors; AS, ammonium sulphate; AD, AS+3, 4-dimethylpyrazole phosphate (DMPP); AN, AS+nitrogen protectant (N-GD); ADN, AS+3, 4-dimethylpyrazole phosphate (DMPP)+nitrogen protectant (N-GD). Error bars represented standard deviations (n = 3).

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Table 2.

Three-way ANOVA (P < 0.05) of soil types (S), nitrification inhibitor (NI) and days after planting (D) and their interactions ((S*NI), (S*D), (NI*D) and (S*NI*D)) on the content of NH4+-N, NO3—N and apparent nitrification rate (ANR).

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Fig 2.

Changes of apparent nitrification rate of three soils during four sampling periods.

Treatment: AS, ammonium sulphate; AD, AS+3, 4-dimethylpyrazole phosphate (DMPP); AN, AS+nitrogen protectant (N-GD); ADN, AS+3, 4-dimethylpyrazole phosphate (DMPP)+nitrogen protectant (N-GD). Error bars represented standard deviations (n = 3).

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Fig 3.

Aboveground biomass of maize under different treatments in three soils.

Treatment: AS, ammonium sulphate; AD, AS+3, 4-dimethylpyrazole phosphate (DMPP); AN, AS+nitrogen protectant (N-GD); ADN, AS+3, 4-dimethylpyrazole phosphate (DMPP)+nitrogen protectant (N-GD). Error bars represented standard deviations (n = 3). Different letters indicate significant differences between different treatments at P < 0.05 by Duncan test.

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Fig 3 Expand

Table 3.

Two-way ANOVA (P < 0.05) of soil types (S) and nitrification inhibitor (NI) on grain yield, agronomic nitrogen use efficiency (ANUE) and apparent nitrogen recovery (AR) of maize.

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Fig 4.

Nitrogen uptake of maize under different treatments in three soils.

Treatment: AS, ammonium sulphate; AD, AS+3, 4-dimethylpyrazole phosphate (DMPP); AN, AS+nitrogen protectant (N-GD); ADN, AS+3, 4-dimethylpyrazole phosphate (DMPP)+nitrogen protectant (N-GD). Error bars represented standard deviations (n = 3). Different letters indicate significant differences between different treatments at P < 0.05 by Duncan test.

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Fig 4 Expand

Fig 5.

Nitrogen use efficiency (ANUE and AR) under different treatments in three soils.

ANUE, agronomic nitrogen use efficiency; AR: Apparent nitrogen recovery. Treatment: AS, ammonium sulphate; AD, AS+3, 4-dimethylpyrazole phosphate (DMPP); AN, AS+nitrogen protectant (N-GD); ADN, AS+3, 4-dimethylpyrazole phosphate (DMPP)+nitrogen protectant (N-GD). Error bars represented standard deviations (n = 3). Different letters indicate significant differences between different treatments at P < 0.05 by Duncan test.

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Fig 5 Expand

Table 4.

Pearson correlation between soil pH, soil organic matter (SOM), NH4+-N and NO3—N.

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Table 4 Expand