Fig 1.
Interface intimate relationship between human MG-63 and microrough titanium implant surfaces with normal or enhanced hydrophilicity.
Human MG-63 were cultured on microrough titanium implant surface with standard (Ti) or enhanced hydrophilicity (hydrophilic Ti), thus prepared and analyzed by fluorescence or by SEM-FIB microscopy. (a) Immunofluorescence staining for actin (red), vinculin (green) and cell nuclei (blue) reveals the polygonal shape of human MG-63 when cultured on titanium surfaces. Scale bar: 50μm. (b) SEM images before and after FIB cross-sectioning reveals the shape and the intimate connection established by the cells (red) with the underlying surface by human MG-63. Scale bar: 2μm. Scale bar close up: 0.2μm. The intimate relationship between cells and titanium was further quantified by measuring cell thickness and the distance occurring between the cell border and titanium. * = p<0.05. Green dashed lines indicate the top and bottom border of the cells, while white stars indicate air bubbles entrapped between cells and titanium.