Fig 1.
The selected seeds of two varieties, CDC Saffron and AC Agassiz, and their number allocation (1 to 10 from small to large).
Fig 2.
A: The standard curve made with a protein standard mix containing bovine thyroglobulin (670 kDa), Blood γ-globulin (150 kDa), ovalbumin (44 kDa), and Ribonuclease A type I-A from bovine pancreas (13 kDa).
B: Size exclusion chromatogram with molecular weight identification of peaks based on the standard curve (Fig 2A) and confirmation of the identifications of 64 and 32 kDa peaks with microfluidic SDS-PAGE.
Fig 3.
The 3D surface plot of pea proteins separated by SEC (X axis) and RP-HPLC (Y axis).
Fig 4.
RP-HPLC areas of six selected peaks from analyzing 10 single seeds of both CDC Saffron and AC Agassiz varieties.
Results are means of three replicates and error bars represent standard deviation. P represents P value for each peak and each variety.
Table 1.
The weight, average diameter, and total protein content of 10 seeds of each variety.
Results are means of three replicates ± standard deviation.
Fig 5.
A: The microfluidic SDS-PAGE gel pictures of extracted proteins from 10 single seeds of both CDC Saffron and AC Agassiz varieties. B: A sample electrogram obtained from the microfluidic SDS-PAGE analysis, including the ladder and identification of peaks based on molecular weights.
Table 2.
Percentage of different protein fractions/subunits for ten individual seeds for two varieties.
Mean, standard deviation, range, CV and P value were provided.