Fig 1.
Expression of (A) MHC class I and (B) MHC class II on 4T1 cells treated with F3. The FACS results were analyzed using the FlowJo v.X.0.7 software (Tree Star Inc., Ashland, OR, USA).
Table 1.
Effects of F3 on the expression of MHC class I and class II in 4T1 cells.
Fig 2.
Representative photomicrographs for expression of MHC class I and class II molecules in untreated and treated tumors.
Arrows indicate positively stained mammary tumor cells. Images were captured at X400 magnification. The scale bar corresponds to 50 μm.
Fig 3.
Absolute counts for expression of MHC class I and class II molecules in treated and untreated tumors.
P values were calculated using Mann-Whitney test for comparison of absolute cell counts between untreated (TM) and treated (TM-F3) tumor-bearing mice.
Table 2.
Effects of F3 on the expression of MHC class I and class II in TM-F3 when compared with the TM group.
Fig 4.
Representative photomicrographs showing expression of CD4, CD8, CD68 and IL-2 in treated and untreated tumors.
Arrows indicate positively stained lymphocytes (CD4 & CD8), macrophages (CD68) and mammary tumor cells (IL2). Images were captured at X400 magnification. The scale bar corresponds to 50 μm.
Fig 5.
Absolute counts for expression of CD4, CD8, CD68 and IL-2 molecules in treated and untreated tumors.
P values were calculated using Mann-Whitney test for comparison of absolute cell counts between untreated (TM) and treated (TM-F3) tumor-bearing mice.
Table 3.
Effects of F3 on the expression of immune markers in TM and TM-F3.