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Table 1.

Demographic and clinical data.

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Fig 1.

Patient distribution in different clinical groups according to the final diagnosis.

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Table 2.

Manufacturer specifications of the compared assays.

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Fig 2.

Passing-Bablok regression.

Correlation between EliA and Elecsys® TRAb. TRAb are displayed in UI/l. Dotted line (- - - -) corresponds to identity. Blue line corresponds to Passing Bablock regression and double dotted line (-- -- --) to 95% CI bands. Tests values are displayed as orange circles.

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Table 3.

Statistical analysis comparing results from Elecsys® and EliA™ assays.

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Fig 3.

Bland-Altman plot.

Inter-assay agreement between EliA and Elecsys® TRAb. The dashed line (........) corresponds to bias. Lower (-8.036) and upper (6.195) 95% limits of agreement are plotted as speckled lines. Y-axis plots the difference scores of EliA and Elecsys® TRAb measurements against the mean for each studied sample.

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Fig 4.

Box-plot of Kruskal-Wallis analysis.

A. Elecsys® TRAb levels in the different groups enrolled in the study. B. EliA™ TRAb levels in the different groups enrolled in the study. TRAb levels are displayed as data ranks obtained by transforming to continue data ranks using Roche’s test. For each box plot, the central line represents the median; the boxes limits represent the upper and lower quartile; black points are outliers (a value more than 1.5 times the interquartile range above/below the interquartile values); and the black lines are the whiskers, which extend from the interquartile ranges to the maximum values that are not classed as outliers.

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Fig 5.

ROC (Receiver operating curve) analysis for EliA™ and Elecsys®.

ROC curve of both methods at manufacturer cut-off values.

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Table 4.

Test performance values obtained from comparative studies for TRAb measurement methods including EliA™ and/or Elecsys® evaluation.

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