Fig 1.
Differential staining and in vitro germination of Cannabis sativa pollen.
(a) Differential staining of non-aborted (pink cytoplasm with blue exines) and aborted pollen grains (blue exines), showing absorption of pink acid fuchsin in the cytoplasm of functional pollen grains. (b) In vitro germination of viable and inviable pollen grains, showing protrusion of the pollen tube in viable pollen grains (blue arrows).
Fig 2.
Pollen grain non-abortion rates under two experimental conditions.
The linear relationship between weeks post anther dehiscence and the non-abortion rate of pollen stored under (a) room temperature conditions (22 ± 0.95°C) and (b) freezer temperature conditions (-4°C).
Fig 3.
Pollen grain viability under room temperature conditions.
The linear relationship between weeks post anther dehiscence and the viability of pools of pollen grains stored under room temperature conditions (22 ± 0.95°C).
Fig 4.
Pollen grain non-abortion rates do not predict viability.
The relationship between viability and the abortion rate of pollen grains from a single sample. The associated linear model has insufficient model fit (Adj. R2 = -0.06, RSE = 17.93); Wilcoxon signed rank analysis determined that the two characteristics were independent, showing no detectable relationship (v = 120, p < 0.001).