Table 1.
Expression of NOI genes in A. thaliana infested with T. urticae.
Table 2.
Expression of NOI genes in A. thaliana infested with different arthropods.
Table 3.
Number of one-NOI and two-NOI RIN4-like/NOI members in different species.
Fig 1.
Phylogenetic tree of RIN4-like/NOI family long two-NOI proteins.
Dicot labels are shown in yellow, monocot in purple, and PAS sequences in orange. The position in the tree of A. thaliana RIN4-like/NOI members upregulated after mite feeding is indicated. Branches are collapsed at 50% bootstrap, values higher than 50% are showed.
Fig 2.
Phylogenetic trees of RIN4-like/NOI family short two-NOI proteins.
A schematic tree for all dicot sequences and the individual group phylogenies are showed. The position in the tree of A. thaliana RIN4-like/NOI members upregulated after mite feeding is indicated. Bootstrap values are included.
Fig 3.
Disorder propensity of RIN4-like/NOI family proteins in different species.
A) Long two-NOI sequences. B) Short one-NOI sequences. Disorder propensity is shown as IUPred score for each amino acid position between 0 and 1, where higher values correspond to a higher probability of disorder. Dicot labels are shown in yellow, monocot in purple, and PAS sequences in orange. Phylogenetic groups in short one-NOI dicot members and the sequences of A. thaliana RIN4-like/NOI members upregulated after mite feeding are indicated.
Fig 4.
Predicted modification sites of RIN4-like/NOI family proteins in different species.
A) Long two-NOI sequences. The group of sequences containing NOI10 and NOI11 is marked with a rectangle. The locations of relevant conserved modifications in this group are shown in blue. B) Recurrence of the predicted modifications per site in long two-NOI dicot sequences, determined as the number of sequences that contains a specific modification. B) Short one-NOI sequences. In A) and B), dicot labels are shown in yellow, monocot in purple, and PAS sequences in orange. Phylogenetic groups of short one-NOI dicot members and the sequences of A. thaliana RIN4-like/NOI members upregulated after mite feeding are indicated.
Fig 5.
Rate per site for long two-NOI sequences (dark green) and short one-NOI sequences (light blue) from dicot species. Partial alignments of the reference sequences, RIN4 for long two-NOI and NOI4 for short one-NOI, with the mite-induced members are shown below the negative evolution rates.
Fig 6.
Structural features of the C-NOI domains of A. thaliana RIN4-like/NOI members.
A) Comparison of RIN4 and the predicted structures of NOI3 and NOI5. B) Comparison of RIN4 and the predicted structures of NOI10, NOI11, and the N-NOI domain of RIN4. Predicted phosphorylated amino acids are shown in sticks.
Fig 7.
A) Scheme (left) and alignment (right) showing the location of the conserved motifs specific for A. thaliana NOI10/NOI11 or shared with RIN4. B) Disorder binding propensity of A. thaliana RIN4, NOI10, and NOI11 amino acids shown as Anchor scores. Numbers mark the approximate location of the conserved motifs described in A).