Table 1.
Drosophila synonyms.
Fig 1.
The complement of ecdysozoan Fox genes.
Modified after [28] (their Fig 2). Green and light green boxes indicate that the gene falls in a given class of Fox genes in all or two of three analyses conducted in [28], respectively. Roman numbers in boxes indicate numbers of paralogs. A dash (-) indicates a predicted loss of a given lineage-specific Fox class gene. Solid vertical grey bars suggest losses of a class of Fox genes. Data on Apis mellifera stem partially from [198]. Note that the lack of FoxJ23 in holometabolous insects in this overview does not represent a general loss in this group of animals (as it is present and some species), and that a possible FoxQ1 gene was identified in a scorpion, albeit with weaker support [28]. A recent report by [62] listed 21 Tribolium Fox genes, but note that this is because three and two identical sequences of FoxO and FoxP respectively were incorporated in their list [62] (their Fig 2). The same analysis lists two Drosophila FoxP genes which appears to be a typo in their figure.
Fig 2.
Expression of Parasteatoda FoxA-2 (A-I) and FoxC (J-O). In all panels, anterior is to the left, ventral view. Panels A, D, G, K, and M view of anterior with head. Panels B, E, H, L, and N view of middle part with walking limbs. Panels C, F, I, O view of opisthosoma. Arrows in panels K and M point to lateral expression in the head. The arrowheads in panels M and N point to faint expression at the ventral rim of the split germ band. Asterisks in panels K and L mark expression in the VNS. Filled circles in panels M-O mark expression at the base of the walking limbs. Abbreviations in Table 2. DAPI staining of the embryos shown in this figure are presented in S6 Fig.
Table 2.
Abbreviations.
Fig 3.
Expression of Euperipatoides FoxC.
In all panels, anterior is to the left, ventral views, except panel D, lateral view, dorsal up. A´ and B´ represent DAPI staining of the embryos shown in A and B. In all panels, arrows point to expression at the posterior margin of the head lobes, and arrowheads indicate expression in the anterior of the jaw-bearing segment. Abbreviations in Table 2.
Fig 4.
Expression of Tribolium FoxD (A-C), FoxF (D-F) and FoxJ1 (G-I). In all panels, anterior is to the left, ventral view. Embryos are flat-mounted. Arrows in panels B and C mark expression in the VNS. Arrows in panels D and E point to the proctodaeum. Arrows in panels G and H mark dorsal expression. Arrowheads in panels D-F mark expression in the heart (dorsal tube). Asterisks in panel I mark dots of expression in dorsal tissue. Abbreviations in Table 2.
Fig 5.
In all panels, anterior is to the left, ventral views. The arrow in panel B marks an appearing stripe of expression in the (likely) mandibular segment primordium. The asterisk in panel E marks the mandibular segment. Arrows in panels J and K mark expression in the VNS. Abbreviations in Table 2.
Fig 6.
Expression of Parasteatoda FoxD.
In all panels, anterior is to the left, ventral views, except panels G, dorsal view and A, H, lateral view. Each row represents the same embryo, except for first row. Arrows point to expression in the VNS. Arrowheads point to expression at the base of the limbs. Abbreviations in Table 2. DAPI staining of the embryos shown in this figure are presented in S10 Fig.
Fig 7.
Expression of Euperipatoides FoxD.
In all panels, anterior is to the left. Panels A, B, and F, ventral view; panels C, E and G, lateral view, panel D, view of the head. A´/B´ represent DAPI staining of the embryos shown in A/B. Abbreviations in Table 2.
Fig 8.
Expression of Glomeris FoxF (A-D), FoxJ1 (E-H) and FoxJ2 (I-L). In all panels, anterior is to the left, ventral views except panel D (lateral view, dorsal up). Asterisks in panel D mark expression in the tergite borders. Arrows in panel H mark dot-like expression in the head. Asterisks in panels K and L mark an area that is free of expression lateral in the head. Abbreviations in Table 2.
Fig 9.
Expression of Parasteatoda FoxF-1 (A-I) and FoxF-2 (J-M). In all panels, anterior is to the left, ventral views, except panels G and M, lateral views, and H, dorsal view. Each row represents the same embryo, if not of different developmental stage. Arrows in panels D-H point to expression in the opisthosoma, and in panels J and K the legs. Abbreviations in Table 2. DAPI staining of the embryos shown in this figure are presented in S12 Fig.
Fig 10.
Expression of Euperipatoides FoxF.
In all panels, anterior is to the left. Panels A and G, ventral view; panels B, D and E, lateral view; panels C and F, dorsal view. A´-D´ and G´ represent DAPI staining of the embryos shown in A-D and G. Arrow in panel D points to expression dorsally abutting the limb buds. Dashed line in panel D indicates dorsal margin of the germ band. Asterisks in panels B and C mark expression at the dorsal rim of the embryo; arrows in B and D point to expression in the dorsal extraembryonic tissue. Arrowheads in panels E and G mark expression in the ventral nervous system. Abbreviations in Table 2.
Fig 11.
Expression of Parasteatoda FoxG.
In all panels, anterior is to the left, ventral views, except panels G, lateral view and H, dorsal view. Each row (except first row, A-C) represents the same embryo. Arrow in panel B marks weak expression in the chelicerae segment. Arrows in panels G and H point to the heart. Abbreviations in Table 2. DAPI staining of the embryos shown in this figure are presented in S13 Fig.
Fig 12.
Expression of Euperipatoides FoxH.
In all panels, anterior is to the left, ventral views. A´/B´ represent DAPI staining of the embryos shown in A/B Abbreviations in Table 2.
Fig 13.
Expression of Parasteatoda FoxJ1 (A-G) and FoxL1 (H-L). In all panels, anterior is to the left, ventral views, except panels D, lateral view, F, dorsal view and G, lateral view. Arrow in panel A marks expression in the chelicera. Arrows in panels E and F point to expression in the spinnerets on opisthosomal segment 5. Asterisks in panels F and G mark a second dot of expression in O5. Arrows in panels H and I point to expression ventral to the base of the limbs. Arrowheads in panels I and L point to expression in the tail region. Abbreviations in Table 2. DAPI staining of the embryos shown in this figure are presented in S15 Fig.
Fig 14.
Expression of Euperipatoides FoxJ2 (A-D) and FoxL (E-G). In all panels, anterior is to the left. Panels A, C and D lateral view. Panels B, and E-G ventral view. A´, D´ and E´-G´ represent DAPI staining of the embryos shown A, D and E-G. Arrow in panel D marks expression ventral to the base of the walking legs. Arrows and arrowheads in panels E-G point to expression at the posterior rim of the head lobes and the posterior pit respectively. Abbreviations in Table 2.
Fig 15.
Expression of Tribolium FoxL1 (A-E) and FoxL2 (F-J). In all panels, anterior is to the left, ventral view. Embryos are flat-mounted, except embryos shown in panels A and F. Arrows in panels B, C, E and J point to expression in the VNS. Short arrow in panel D marks a ring of expression at the base of the Malpighian tubules. Note the unspecific staining of the pleuropodia in panels D, E, and J. Abbreviations in Table 2.
Fig 16.
Expression of Glomeris FoxL1 (A-D), FoxL2 (E-H), and FoxP (I-L). In all panels, anterior is to the left, ventral views (except panel H, lateral view). Arrows in panels B and C point to expression in the developing midgut. Arrowheads in panel G point to two domains of expression in a fusing dorsal segmental unit. Roman numerals mark expression in the dorsal segmental units (cf. Janssen 2011). The arrow in panel K points to expression in the VNS. The arrow in panel L points to expression in the last formed segment. Abbreviations in Table 2.
Fig 17.
Expression of Tribolium FoxO (A-H) and FoxP (I-K). In all panels, anterior is to the left, ventral view; except panel F, lateral view. In panel G, anterior is up. Embryos in panels E, H, J and K are flat-mounted. Long arrows in panels C and D mark the posterior border of strong expression in the head. Short arrows in panel C mark posterior border of expression in the extraembryonic tissue. Asterisks in panel H mark expression in the lateral tissue of the trunk segments. Filled circles in panel H mark dot-like expression in the VNS. Arrows in panels J and K point to expression in the VNS. Note the unspecific staining of the pleuropodia in panels H and K. Abbreviations in Table 2.
Fig 18.
Expression of Parasteatoda FoxO-1 (A-I) and FoxO-2 (J-R). In all panels, anterior is to the left, ventral views, except panels D, F, G, I, and R (lateral views), and H (dorsal view). Each row represents the same embryo, except panels G and H. Arrows in panels A, D, E and F point to expression in the interface between the embryo proper and the dorsal field. Arrow in panel J points to dot-like expression in the brain. Arrows in panels K and L point to expression in the VNS. Arrowheads in panels M and P point to lateral expression in the head lobes. Filled circles in panels M and P mark expression anterior in to the mouth. The asterisk in panel O points to strong expression in the VNS of nascent segments; the arrow in panel O points to weaker expression anterior to that. Arrowhead in N points to dot-like expression dorsal to the base of the walking legs. Abbreviations in Table 2. DAPI staining of the embryos shown in this figure are presented in S16 Fig.
Fig 19.
Expression of Euperipatoides FoxO (A-F) and FoxP (G, H). In all panels, anterior is to the left. Panels A, B, E and H show ventral views; panels C, D, F, and G show lateral views. Arrow in panel B point to expression at the anterior rim of the head lobe. Arrowhead in panel B point to a segmentation gene like ventral stripe of expression in the last formed posterior segment. Abbreviations in Table 2.
Fig 20.
Expression of Parasteatoda FoxP-1 (A-C) and FoxP-2 (D-K). In all panels, anterior is to the left. Panels A and B show lateral view; panel C and L show dorsal views. All other panels show ventral views. Dotted lines mark the area of expression in the dorsal field. Arrowheads in panels G and H point to expression in the VNS. Arrows in panel J point to dot-like expression at the dorsal edge of the embryo. Abbreviations in Table 2. DAPI staining of the embryos shown in this figure are presented in S17 Fig.
Fig 21.
Panel A ventral view, panel B lateral view and panel C close-up on head, dorsal view. Note the unspecific staining in the pleuropodia in panels A and B. Abbreviations in Table 2.