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Fig 1.

PCV13 vaccination schedule and sampling strategy.

A. PCV13 vaccination schedule based on age. B. Overview of sampling strategy. Blood samples were taken during both the 1st and the 2nd sampling moment. In the pre-vaccination serum samples (1st sampling) PCV13 IgG levels and metabolic hormones were determined. In the post-vaccination serum samples (2nd sampling) only PCV13 IgG levels were determined. * Median 6.7 weeks (IQR 6.4–6.9 weeks).

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Fig 1 Expand

Table 1.

Baseline characteristics of the study population.

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Table 1 Expand

Fig 2.

A: Correlation between adiponectin and post vaccination pneumococcal IgG antibody concentrations. Correlation, shown by a linear regression line, between pre-vaccination adiponectin levels and post-vaccination log-transformed pneumococcal IgG antibody concentrations for stunted, normal weight and overweight children. r = Pearson correlation coefficient. *p = <0.05. $ P-value value of interaction term in multivariable regression model (Table 3). B: Correlation between ghrelin and post vaccination pneumococcal IgG antibody concentrations. Correlation, shown by a linear regression line, between pre-vaccination ghrelin levels and post-vaccination log-transformed pneumococcal IgG antibody concentrations for stunted, normal weight and overweight children. r = Pearson correlation coefficient. $ P-value of interaction term in multivariable regression model (Table 3). C: Correlation between leptin and post vaccination pneumococcal IgG antibody concentrations. Correlation, shown by a linear regression line, between pre-vaccination leptin levels and post-vaccination log-transformed pneumococcal IgG antibody concentrations for stunted, normal weight and overweight children. r = Pearson correlation coefficient. *p = <0.05. $ P-value of interaction term in multivariable regression model (Table 3).

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Fig 2 Expand

Table 2.

Stratified univariate analysis of the effect of metabolic hormones on vaccine response in children with varying nutritional status.

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Table 2 Expand

Table 3.

Results from multivariable linear GEE models for post-PCV13 serum antibody levels in stunted and overweight children.

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Table 3 Expand