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Fig 1.

A gutter cluttered with Theba pisana behind a local business in Oceanside, CA (A). A utility box covered with aestevating T. pisana beside a local business in Oceanside, CA (B). Clusters of aestivating T. pisana on a tree beside a sidewalk in Oceanside, CA (C).

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Fig 1 Expand

Fig 2.

Treatment arena for determining the lethality of three US strains of Phasmarhabditis californica (ITD726), P. papillosa (ITD510), and P. hermaphrodita (ITD272) against (11.5-15mm/0.4–1.2 gram) Theba pisana at 5 times recommended dose of 150IJs/cm2 (A) and for the dosage dependence of P. californica lethality against (11.5-15mm/0.5–1.3 gram) T. pisana at 30 IJs/cm2 (Nemaslug® recommended dose) and 90 IJs/cm2 (B).

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Fig 2 Expand

Fig 3.

A dead adult Theba pisana snail with mixed stages of Phasmarhabditis californica (ITD726) within the shell cavity.

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Fig 3 Expand

Fig 4.

Kaplan Meier graph showing the percent survival of large adult Theba pisana over 14 days after exposure to 5 times the Nemaslug ®-recommended dose (150 IJs/cm2) of three US strains of Phasmarhabditis californica (ITD726), P. papillosa (ITD510), and P. hermaphrodita (ITD271), and Sluggo Plus®.

The snails only control included a treatment with no application of Phasmarhabditis. **** indicates a p value less than 0.0001 compared to untreated control. Statistical analyses were performed by doing Mantel-Cox log rank analyses comparing each treatment to each other.

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Fig 4 Expand

Fig 5.

Kaplan Meier graph showing the percent survival of adult Theba pisana over 14 days after exposure to the Nemaslug ®-recommended dose (30 IJs/cm2) and three times the recommended dose (90 IJs/cm2) of Phasmarhabditis californica (ITD726) and Sluggo Plus®.

** indicates a p value less than 0.01 compared to the untreated (snails only) control. Statistical analyses were performed by doing Mantel-Cox log rank analyses comparing each treatment to each other.

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Fig 5 Expand