Table 1.
Demographic and microbiological data of included cats.
Table 2.
List of the 15 most expressed miRNAs in feline urine using small RNAseq.
Fig 1.
Venn diagram of the differentially abundant (DA) miRNAs in each of the analyzed comparisons.
A. Shared and private miRNAs when comparing healthy cats (Control) with other pathologic urological conditions. In brackets, the total number of DA miRNAs in each comparison between healthy cats (Control) and cats with PN, SB/C, UO or CKD. B. Shared and private miRNAs when comparing cats with PN and cats with other urological conditions. In brackets, the total number of DA miRNAs in each comparison between PN cats and cats with SB/C, UO, CKD and all these disease states together (SB/C+UO+CKD). The miRNAs found to be shared or private in each defined comparison are provided within each comparison with a superindex letter. miRNAs shown in a squared box and highlighted in black correspond to DA miRNAs specific for each group (private). In light grey, the DA miRNAs shared between 2 or more of the defined contrasts. CKD: Chronic Kidney Disease; PN: Pyelonephritis; SB/C: Subclinical Bacteriuria /Cystitis; UO: Ureteral Obstruction.
Table 3.
Differentially abundant (DA) miRNAs from qPCR analyses.
Fig 2.
Box-plot of differentially abundant (DA) miRNAs between healthy Control cats and each of the defined groups by using qPCR.
Selected miRNAs detected as DA (|log2FC| ≥ 1.5; p-value < 0.05) are shown. Cats with culture-positive urine after cystocentesis are highlighted in red (E. coli), blue (Staphylococcus spp.) and green (other pathogens), while cats with culture-negative urine are depicted in black. ***: DA miRNAs with q-value < 0.01. **: DA miRNAs with q-value > 0.01 & < 0.05. *: DA miRNAs with p-value < 0.05 but not significant after multiple testing correction (q-value > 0.05). PN: Pyelonephritis, SB/C: Subclinical bacteriuria/Cystitis, UO: Ureteral obstruction, CKD: Chronic kidney disease.
Fig 3.
UMAP plot depicting sample clustering of a collection of tissues from the canine miRNA atlas and the feline urinary samples analyzed in the current study.
The plot includes dog expression miRNA profiles from brain, colon, duodenum, jejunum, ileum, plasma, heart, skeletal muscle, skin, kidney, liver, lung and pancreas (round shape), as well as the predicted projection of the feline urinary samples using small RNAseq data (named Urine, diamond shape), including healthy cats (Control, n = 8) and cats with urological pathological conditions (PN, SB/C, UO or CKD, n = 27).