Fig 1.
Synthesis of T/AgNPs by plant extract.
Table 1.
Primers sequences, target genes, amplicon sizes and cycling conditions.
Fig 2.
UV-vis spectra of plant extract (green) and silver nanoparticles (red).
Fig 3.
FTIR spectrum of plant extract (red) and silver nanoparticles (green).
Fig 4.
HRTEM image of silver nanoparticles.
Fig 5.
AFM images of Thyme based silver nanoparticles (T/AgNPs).
“The AFM images display dense aggregates of quasi-spherical AgNPs with an average size of 22 nm. The aggregation shown in AFM images is attributed to the sampling process for AFM leading to dropping a few drops of NPs solution on a silica glass plate and allowing drying to get a thin film of NPs. This will allow the NPs to be clustered together. The AFM images confirm the narrow particle size distribution obtained from TEM images”.
Fig 6.
Selected disks show the disk diffusion (antibacterial) activity of silver nanoparticles.
(A) Inhibition zone present around the disc dipped in AgNPs and (B) inhibition zone absent around the disc (control).
Fig 7.
TEM images of the effect of Thymus vulgaris silver nanoparticles on Candida; destruction of the fungal cell wall with disruption of membrane.
Fig 8.
TEM images of SIL (Solid Immersion Lens) showing shrinkage of Candida albicans isolates after addition of biologically active silver nitrate particles.
Fig 9.
TEM images showing intracellular biologically active T/AgNPs and cellular disruption.
Fig 10.
TEM images of the effect of biologically active silver nitrate on Candida albicans isolates (image contains disrupted cells).
Fig 11.
TEM images of intracellular and extracellular distribution of biologically active silver nitrate (measured cells are disrupted).
Fig 12.
(A) The percentage of resistant isolates to chemical antifungal agents and T/AgNPs. (B) Anti-fungal sensitivity testing of commercial antifungal versus biogenic silver nitrate.
Fig 13.
Gel electrophoresis of Candida virulence genes, Secreted Aspartic Protease gene (SAP4) was detected at 394 bp, RAS1 was detected at 106 bp, Hyphal-Associated Adhesin (HYR1) gene was detected at 243 bp and agglutinin-like sequence gene (ALS3) was detected at 122 bp.
Table 2.
Demographic, clinical-laboratory data, and prognosis of patient groups.
Table 3.
The sociodemographic, laboratory, antifungal sensitivity, virulence factors in Candida albicans versus Candida non-albicans isolates.