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Fig 1.

The expression level of COL1A1 in normal and tumor tissues.

(A) In the TIMER database, the expression level of COL1A1 in different cancer tissues compared with normal tissues. (B) the expression level of COL1A1 in GEO. (C-E) The expression level of COL1A1 in different subtypes of glioma (A: Astrocytoma, O: Oligodendro, rO: recurrent Oligodendro, rA: recurrent Astrocytoma, AA: Anaplastic Astrocytoma, AO: Anaplastic Oligodendro, rAA: recurrent Anaplastic Astrocytoma, rAO: recurrent Anaplastic Oligodendro, GBM:Glioblastoma, rGBM: recurrent Glioblastoma.** P < 0.05,** P < 0.005, *** P < 0.001).

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Fig 1 Expand

Fig 2.

Overall survival curve of high and low expression of COL1A1 in glioma and it’s subclass.

(A) Survival curves of OS by Kaplan-Meier respectively in LGG. (B-E) Survival curves of Survival probability in Mutant, Wildtype, Non-codel and Codel. (F-G) Survival curves of DSS and PFI in LGG. DSS, disease specific survival; PFI, progression free interval.

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Fig 2 Expand

Fig 3.

Survival curves and cox proportional hazards regression model of DFI, DSS, and PFI in different types of cancers.

(A-C) Cox proportional hazards regression model of DFI, DSS, and PFI. (D) Cox proportional hazards model of OS in LGG for expression of COL1A1. DFI, disease free interval.

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Fig 3 Expand

Fig 4.

The expression of COL1A1 in LGG grade and the forest plot of LGG.

A. Differential expression of COL1A1 in different cancer grade of LGG. Based on data from 258 grade2(G2) patients and 270 grade3(G3) patients in TCGA. B. Multivariate Cox analysis of COL1A1 expression and clinical pathological factors. As age, tumor grade and COL1A1 expression are independent prognostic factors (* P <0.05; ** P < 0.005;*** P < 0.001).

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Fig 4 Expand

Table 1.

Univariate (a) and multivariate analysis (b) of the correlation of COL1A1 expression with OS among LGG patients.

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Table 1 Expand

Fig 5.

Radar graph for relationship between the expression of COL1A1 and TMB of each tumor sample.

Correlation between Tumor mutational burden and LGG, THYM, PRAD, LUAD, LAML and KICH(* P <0.05; ** P < 0.005;*** P < 0.001).

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Fig 5 Expand

Fig 6.

Correlation between COL1A1 expression and TME in diffrent tumer.

(A-C). Relationship between COL1A1 expression and Stromal Score, Immune Score and ESTIMATE Score in BLCA. (D-F). Correlation between COL1A1 expression and Stromal Score, Immune Score and ESTIMATE Score in LGG. (G-I). Relationship between COL1A1 expression and TME in LIHC.

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Fig 6 Expand

Table 2.

Correlation analysis of COL1A1 expression level and TME score.

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Table 2 Expand

Fig 7.

Immune infiltration level and survival time in LGG.

A. Correlation of COL1A1 expression with infiltrating levels of B cell, CD4+ T cells, CD8+ T cells, Macrophages, Neutrophils and DCs in LGG. B. Cumulative survival of COL1A1 expression related to B cell, T cells, Macrophages, Neutrophils and DCs in LGG.

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Fig 7 Expand

Table 3.

Correlation analysis of COL1A1 expression level and immune cell markers in TIMER.

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Table 3 Expand

Fig 8.

Correlation of the expression between COL1A1 and immune checkpoint genes in different tumors.

Based on Pearson correlation coefficients, COL1A1 has a significant correlation with most selected immune checkpoint genes in LGG(* P <0.05; ** P < 0.005;*** P < 0.001).

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Fig 8 Expand