Fig 1.
Distribution and proportion of staphylococci from different sites of sampling from 116 dogs attended at two different veterinary clinics from Belo Horizonte, Brazil, between 2017 and 2020.
Disclosure—The recently approved differentiation of S. schleiferi into two species, S. coagulans and S. schleiferi [40], was not considered in the present work.
Table 1.
Distribution and proportion of staphylococci species isolated from different sites of sampling from 116 dogs at two different veterinary clinics from Belo Horizonte, Brazil, between 2017 and 2020.
Fig 2.
Antimicrobial resistance among clinical S. pseudintermedius isolates from infected dogs from two different veterinary clinics from 2017 to 2020 in Belo Horizonte, Brazil.
a) Overall prevalence according to the antimicrobial compound; b) According to site of infection.
Table 2.
Antimicrobial resistance among staphylococci isolates from infected dogs attended at two different veterinary clinics, from 2017 to 2020, in Belo Horizonte, Brazil.
Table 3.
Antimicrobial resistance profile of methicillin-resistant S. pseudintermedius (MRSP) strains isolated from dogs (Belo Horizonte, Brazil) according to sequence types (STs) and infection sites.
Fig 3.
Genetic relationship of MRSP from dogs in Belo Horizonte (2017–2020).
a) Population snapshot of MRSP goeBURST full MST analysis. Line numbers and shading indicate the number of differing loci between STs and colors indicate the type of infection related to each ST. b) A phylogenetic tree based on the alignment of all MLST genes of each ST was inferred using iqTree; posterior probabilities are shown in red. Columns with triangles, circle and star indicate the site of infection of each isolate; colored squares’ columns indicate presence or absence of resistance to different classes of non-beta-lactams antibiotics.
Fig 4.
goeBURST population snapshot of S. pseudintermedius at single locus variance (SLV).
Blue color indicates worldwide strain types, while red color indicates MRSP strains identified in the present study; line numbers indicate allelic variance. a) Major populational structure of S. pseudintermedius. Clusters of linked isolates correspond to clonal complexes (CCs); singletons are not showed. Gray boxes indicate the localization of STs identified in the current study. b) snapshot of CC2166, with ST 2124 in red. c) snapshot of CC1758, with STs 2129 and 2164 in red. d) snapshot of CC781 and adjacent, with STs 2125, 2126, 2127, 2128 and 2131 in red. e) Snapshot of CC71 with STs 71 and 2130 in red.