Fig 1.
The stemness signature gene set intersects with previously published ESC signature gene sets.
The number of genes in the intersection between sets on the X and Y axes is shown in the cells of the matrix. The color scale encodes the size of the intersection relative to the size of the dataset on the Y axis (above the diagonal) and relative to the size of the dataset on the X axis (below the diagonal).
Fig 2.
Principal Component Analysis (PCA) of the expression of stemness signatures (A), proliferation signatures (B), and genes representing EMT-MET (C) in differentiating iPSCs, ESCs, ASCs, tumors and normal tissues shown as individual samples (top) and density (bottom).
Tumors span continuously between normal tissues and iPSCs in the expression subspaces of stemness, proliferation, but not EMT-MET genes. Tumor samples form two clusters: one closer to ESCs and iPSCs, another closer to the normal tissues and ASCs.
Fig 3.
Heterogeneity of stemness and proliferation signatures across tumor types.
The first principal component (PC1) encodes the signature of stemness (A), proliferation (B), and EMT-MET signature genes (C). The samples are plotted separately for each tumor in the TCGA project, normal tissues, and iPSCs. Tumor samples are colored according to their position relative to the PC1 median (black vertical line). Tumor samples located to the right (left) of the median are assigned to the Normal (Stem) cluster, respectively. (D) The standard TCGA notation for cancer types.
Fig 4.
The relationship between stemness and proliferation intensities.
(A) Stemness intensity vs. proliferation intensity in 19 solid tumors with different average project-wise tumor stage and median project-wise purity. (B) A heatmap of stemness and proliferation intensities across 19 solid tumors from TCGA. Two groups of tumor samples are identified: top-right quadrant (stemness Intensity>0.5 and proliferation Intensity>0.5) and bottom-left quadrant (stemness Intensity<0.5 and proliferation Intensity<0.5). The notation for cancer types as in Fig 3. Tumors from stem cell rich organs are marked with asterisks.
Fig 5.
Intertumor heterogeneity of stemness and proliferation signatures.
(A) Stem and Normal clusters specific to each tumor type are defined by the location of a sample relative to the median PC1 value across all samples of the given tumor (plotted as horizontal black lines). Samples above (below) the median are assigned to the Normal (Stem) cluster, respectively. (B) Hazard ratios for the comparison of patient survival in Stem and Normal clusters in two sample groups (top-right and bottom-left in Fig 4) with respect to stemness and proliferation intensity. Error bars denote 95% confidence intervals. Tumor types are annotated at the bottom according to the average tumor stage and the median tumor purity. Codes for statistical significance reported are as follows: NS − p-value >0.05, * − 0.01< p-value <0.05, ** − 0.001< p-value <0.01, *** − p-value <0.001. The notation for cancer types as in Fig 3.
Fig 6.
Intertumor heterogeneity of stemness and proliferation is predictive of poor survival.
Kaplan-Meier survival curves grouped by clustering according to stemness (proliferation) signature are plotted for skin melanomas (A), lung adenocarcinoma (B) and renal clear cell carcinoma (C). P-values from the logrank test are reported. The notation for cancer types as in Fig 3.
Fig 7.
Cancer cells show substantial intratumor heterogeneity of stemness.
(A) UMAP dimensionality reduction of the colorectal cancer single-cell RNA-seq dataset. The annotation of normal and cancer cell clusters is from [38]. The cells are colored by the degree of stemness signature reactivation (the absolute value of PC1 projection). (B) The distribution of stemness signature (PC1) (X axis) in normal and cancer cells.
Fig 8.
Cancer cells show heterogeneity in stemness, proliferation and EMT scores.
(A) Stemness, proliferation and EMT scores in single cells by cell type. Boxplots colors correspond to normal (blue), colorectal cancer (orange), and stem (red) cells. (B) Two dimensional embedding derived from UMAP colored by the stemness score from panel A. (C) Stemness scores computed for single cells from primary lung adenocarcinomas (orange), metastatic lesions (red), and surrounding normal tissues (blue). (D) Stemness scores of colorectal cancer cells from different patients.